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首页> 外文期刊>The Biochemical Journal >An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels
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An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels

机译：由肌醇Tris /四磷酸激酶1（ITPK1）和肌醇五磷酸二磷酸二磷脂二磷磷脂磷酸盐水平（ITPK1）和肌醇五磷酸三磷酸三磷酸二磷酸盒（ITPK1）和肌醇五磷酸二磷酸二磷酸盒（IPK1）组成

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摘要

Inositol polyphosphates are ubiquitous molecular signals in metazoans, as are their pyrophosphorylated derivatives that bear a so-called 'high-energy' phosphoanhydride bond. A structural rationale is provided for the ability of Arabidopsis inositol tris/tetrakisphosphate kinase 1 to discriminate between symmetric and enantiomeric substrates in the production of diverse symmetric and asymmetric myo-inositol phosphate and diphospho-myo-inositol phosphate (inositol pyrophosphate) products. Simple tools are applied to chromatographic resolution and detection of known and novel diphosphoinositol phosphates without resort to radiolabeling approaches. It is shown that inositol tris/tetrakisphosphate kinase 1 and inositol pentakisphosphate 2-kinase comprise a reversible metabolic cassette converting Ins(3,4,5,6)P-4 into 5-InsP(7) and back in a nucleotide-dependent manner. Thus, inositol tris/tetrakisphosphate kinase 1 is a nexus of bioenergetics status and inositol polyphosphate/diphosphoinositol phosphate metabolism. As such, it commands a role in plants that evolution has assigned to a different class of enzyme in mammalian cells. The findings and the methods described will enable a full appraisal of the role of diphosphoinositol phosphates in plants and particularly the relative contribution of reversible inositol phosphate hydroxykinase and inositol phosphate phosphokinase activities to plant physiology.
机译：肌醇多磷酸盐是普遍存在的聚酰亚胺中的分子信号，与其具有所谓的“高能量”磷酸氢键键的焦磷酸化衍生物是具有所谓的“高能量”的衍生物。提供了拟南芥肌醇Tris /四磷酸激酶1在不同对称和不对称肌醇磷酸盐和二磷肌醇磷酸盐（肌醇焦磷酸盐）产物中的对称和对映体基材之间区分对称和对映体基材的能力的结构理由。简单的工具应用于色谱分辨率和检测的已知和新的二磷肌醇磷酸盐，而无需采用放射性标记方法。结果表明，肌醇三/四磷酸激酶1和肌醇五磷酸二磷酸2-激酶包含可逆代谢盒将INS（3,4,5,6）P-4转化为5 isp（7）并以核苷酸依赖的方式返回。因此，肌醇三/四磷酸磷酸激酶1是生物植物地位和肌醇多磷酸盐/二磷膦酸性磷酸盐代谢的Nexus。因此，它命令在植物中发挥作用，其在哺乳动物细胞中分配给不同类别的酶。所描述的发现和方法将能够完全评估二磷肌醇磷酸盐在植物中的作用，特别是可逆肌醇磷酸盐羟基胰酶和肌醇磷酸磷酸氨基酶活性的相对贡献植物生理学。

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  • 来源
    《The Biochemical Journal》 |2020年第14期|共18页
  • 作者

    Whitfield Hayley; White Gaye; Sprigg Colleen; Riley Andrew M.; Potter Barry V. L.; Hemmings Andrew M.; Brearley Charles A.;

  • 作者单位

    UEA Sch Biol Sci Norwich Res Pk Norwich NR4 7TJ Norfolk England;

    UEA Sch Biol Sci Norwich Res Pk Norwich NR4 7TJ Norfolk England;

    UEA Sch Biol Sci Norwich Res Pk Norwich NR4 7TJ Norfolk England;

    Univ Oxford Dept Pharmacol Med Chem &

    Drug Discovery Mansfield Rd Oxford 0X1 3QT England;

    Univ Oxford Dept Pharmacol Med Chem &

    Drug Discovery Mansfield Rd Oxford 0X1 3QT England;

    UEA Sch Biol Sci Norwich Res Pk Norwich NR4 7TJ Norfolk England;

    UEA Sch Biol Sci Norwich Res Pk Norwich NR4 7TJ Norfolk England;

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  • 正文语种 eng
  • 中图分类 生物化学;
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