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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Evaluation of two in vitro test systems employing Brugia malayi parasite for prescreening of potential antifilarials.
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Evaluation of two in vitro test systems employing Brugia malayi parasite for prescreening of potential antifilarials.

机译：评估两个采用马来氏布鲁氏菌寄生虫的体外测试系统，以对潜在的抗丝虫剂进行预筛选。

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摘要

A total of 38 new synthetic compounds and 19 plant products that were found active or inactive in Acanthocheilonema viteae-Mastomys coucha [Praomys coucha] and/or Brugia malayi-M. coucha models were tested in in vitro systems using inhibition of B.malayi adult and microfilarial motility (motility assay) and inhibition of MTT (3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction potential (MTT assay) of the parasite as test parameters. Ivermectin and diethylcarbamazine were included as standards. All the synthetic and plant products that were active in the B. malayi-M. coucha model were also active in the in vitro systems. About 82% of the synthetic products and 20% of the plant products that were active in the A. viteae-M. coucha system were also active in the in vitro tests. In addition, 87.5% of the synthetic products and 9% of the plant products that were inactive in the A. viteae-M. coucha system were active in the in vitro tests. It is suggested that plant products showing LC100 in the range of 31.25 鎔/ml to 62.5 鎔/ml in the in vitro tests can be considered as potential antifilarials and followed-up in in vivo assay systems. It is concluded that the motility and MTT assays using both the adults and microfilariae of B.malayi are reliable prescreens with high predictive value; both the assays are necessary for screening synthetic compounds whereas the motility assay using adult worms alone appears sufficient for screening plant products.
机译：总共发现了38种新合成化合物和19种植物产品，这些蛋白在棘皮棘皮动物-乳头草[Praomys coucha]和/或马来酸Brugia-M中有活性或无活性。在体外系统中测试了bedca模型，使用了对B.malayi成虫和微丝虫运动性的抑制（运动性测定）和对MTT（3-（4,5二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑）还原电位的抑制作用（MTT分析）的寄生虫作为测试参数。伊维菌素和二乙基卡巴马嗪被纳入标准。在马来西亚芽孢杆菌中活跃的所有合成和植物产品。 Coucha模型在体外系统中也很活跃。在viteae-M中有活性的约82％的合成产品和20％的植物产品。 Coucha系统在体外测试中也很活跃。此外，在A. viteae-M中没有活性的87.5％的合成产品和9％的植物产品。长沙发系统在体外测试中很活跃。建议将在体外测试中显示LC100在31.25镕/ ml至62.5镕/ ml范围内的植物产品视为潜在的抗丝虫剂，并应在体内测定系统中进行后续跟踪。结论是，使用成人和微丝B虫的运动性和MTT检测是可靠的预筛查方法，具有较高的预测价值。两种测定法都是筛选合成化合物所必需的，而仅使用成虫的活力测定法似乎足以筛选植物产品。

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