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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Direct somatic embryogenesis from zygotic embryos of a timber-yieldingleguminous tree, Hardwickia binata Roxb.
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Direct somatic embryogenesis from zygotic embryos of a timber-yieldingleguminous tree, Hardwickia binata Roxb.

机译：直接从木材产量的豆科树Hardwickia binata Roxb的合子胚中进行体细胞胚发生。

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摘要

We report here direct somatic embryogenesis from semi-mature zygotic embryos (60-75 days after anthesis) of a timber-yielding leguminous tree, Hardwickia binata Roxb,, commonly known as Anjan. High frequency direct somatic embryogenesis was achieved on Murashige and Skoog's (MS) medium supplemented with 2.26 muM 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos proliferated rapidly in subsequent cultures, Various developmental stages were observed during the maturation of somatic embryos, including globular, heart-shaped, torpedo and cotyledonary stages. Maturation of somatic embryos was achieved by transferring isolated somatic embryos to Ms medium containing 0.26 muM alpha -naphthaleneacetic acid (NAA) and 2.22 muM 6-benzylaminopurine (BAP) or 1.89 muM abscisic acid (ABA). In long-term cultures, secondary somatic embryos developed from isolated torpedo-shaped and cotyledonary embryos. Bipolarity of the somatic embryos was confirmed by histological studies.
机译：我们在这里报告从半熟的合子胚（花期后60-75天）的一种直接产生的体细胞胚胎发生，这种半合子胚是产木材的豆科树Hardwickia binata Roxb，通常被称为Anjan。在补充了2.26μM2,4-二氯苯氧基乙酸（2,4-D）的Murashige和Skoog（MS）培养基上实现了高频直接体细胞胚发生。体细胞胚在随后的培养中迅速增殖。在体细胞胚成熟期间，观察到各种发育阶段，包括球形，心形，鱼雷和子叶阶段。体细胞胚的成熟是通过将分离的体细胞胚转移到含有0.26μMα-萘乙酸（NAA）和2.22μM6-苄氨基嘌呤（BAP）或1.89μM脱落酸（ABA）的Ms培养基中来实现的。在长期培养中，次生体细胞胚由分离的鱼雷形和子叶胚发育而来。组织学研究证实了体细胞胚胎的双极性。

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