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Genomics, DNA chips and a revolution in plant biology

机译:基因组学,DNA芯片与植物生物学的一场革命

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The article gives an overview of current research on plant genomics. We begin with a brief account of the major techniques developed for the Human Genome Project such as preparation of RFLP maps, making of cosmid and YAC (later also BAC and PAC) libraries, construction of contigs and finally sequencing such as by use of M13 vectors and use of modern machines and advanced computers. We then introduce to the reader the more recent work with Arabidopsis and rice genomes utilizing the various basic techniques. The article ends with a brief account of the new DNA microchip technology, whereby one can simultaneously monitor expression of hundreds and thousands of genes. Microchips thus are the hub of a new revolution in functional genomics.
机译:本文概述了当前有关植物基因组学的研究。我们首先简要介绍为人类基因组计划开发的主要技术,例如准备RFLP图谱,制作粘粒和YAC(后来也称为BAC和PAC)文库,构建重叠群,最后进行测序(例如使用M13载体)以及现代机器和先进计算机的使用。然后,我们向读者介绍利用各种基本技术进行的拟南芥和水稻基因组的最新研究。本文最后简要介绍了新的DNA微芯片技术,从而可以同时监视成百上千个基因的表达。因此,微芯片是功能基因组学新革命的中心。

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