Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L.

Sanjukta Parui; Sudhendu Mandal; Parui S; Mandal S

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Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L.

机译：曼陀罗变态反应性花粉的生化分析和皮肤敏感性测试。

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Quantitative estimation of carbohydrates, lipids, proteins and free amino acids, in addition to the identification of free amino acids by paper chromatography and TLC, were carried out to determine the chemical composition of pollen collected from Datura metel plants in and around Santiniketan, India. The protein profile was studied by SDS-PAGE, while skin prick tests with the pollen antigen were conducted on 98 patients suffering from rhinitis or bronchial asthma, as well as on healthy volunteers.The pollen of D. metel showed high protein contents (22%) and high concentrations of proline, amino-n-butyric acid and arginine. SDS-PAGE of pollen protein showed 12 bands ranging between 18 and 127.2 kDa. In skin tests, 29% of patients showed a positive response to the antigen.

机译：除了通过纸色谱和TLC鉴定游离氨基酸外，还对碳水化合物，脂质，蛋白质和游离氨基酸进行了定量估计，以确定从印度Santiniketan及其附近的曼陀罗梅特尔植物中采集的花粉的化学成分。通过SDS-PAGE对蛋白质谱进行了研究，同时对98例患有鼻炎或支气管哮喘的患者以及健康志愿者进行了使用花粉抗原的皮肤点刺试验。梅特氏螺旋体的花粉显示出较高的蛋白质含量（22％）和高浓度的脯氨酸，氨基正丁酸和精氨酸。花粉蛋白的SDS-PAGE显示12条带，范围在18至127.2 kDa之间。在皮肤测试中，有29％的患者对该抗原呈阳性反应。

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《Current Science: A Fortnightly Journal of Research》 |1998年第1期|共3页
作者
Sanjukta Parui; Sudhendu Mandal; Parui S; Mandal S;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
allergens; pollen; allergies; chemical composition; carbohydrates; lipids; plant composition; amino acids; chromatography; proteins; antigens; SDS PAGE; rhinitis; asthma; proline; arginine; allergic reactions; weeds; Datura metel; man;

机译：过敏原;花粉;过敏;化学成分;碳水化合物;脂质;植物成分;氨基酸;色谱法;蛋白质;抗原;SDS PAGE;鼻炎;哮喘;脯氨酸;精氨酸;过敏反应;杂草;曼陀罗;

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1. Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L. [J] . Sanjukta Parui, Sudhendu Mandal, Parui S, Current Science: A Fortnightly Journal of Research . 1998,第1期

机译：曼陀罗变态反应性花粉的生化分析和皮肤敏感性测试。
2. Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L. [J] . Mandal Sudhendu, Parul Sanjukta Current science . 1998,第01期

机译：曼陀罗变态反应性花粉的生化分析和皮肤敏感性测试。
3. Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L. [J] . Mandal Sudhendu, Parul Sanjukta Current science . 1998,第01期

机译：曼陀罗变态反应性花粉的生化分析和皮肤敏感性测试。
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机译：不同群体形态学和植物化学成分的比较分析及达尔义林植物的不同群体。和旁遮普平原的Datura Metel L.

Biochemical analysis and skin sensitivity test of the allergenic pollen of Datura metel L.

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