Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells

Maekawa Yuki; Onishi Akishi; Matsushita Keizo; Koide Naoshi; Mandai Michiko; Suzuma Kiyoshi; Kitaoka Takashi; Kuwahara Atsushi; Ozone Chikafumi; Nakano Tokushige; Eiraku Mototsugu; Takahashi Masayo

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Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells

机译：优化的培养系统可诱导从小鼠和人类胚胎干细胞分化出来的三维视网膜聚集体中视网膜神经节细胞的神经突生长。

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Purpose: To establish a practical research tool for studying the pathogenesis of retinal ganglion cell (RGC) diseases, we optimized culture procedures to induce neurite outgrowth from three-dimensional self-organizing optic vesicles (3D-retinas) differentiated in vitro from mouse and human embryonic stem cells (ESCs).

机译：目的：为了建立一种实用的研究工具来研究视网膜神经节细胞（RGC）疾病的发病机理，我们优化了培养程序，以诱导从小鼠和人类体外分化出的三维自组织视神经小泡（3D-视网膜）中的神经突向外生长。胚胎干细胞（ESC）。

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《Current Eye Research》 |2016年第4期|共11页
作者
Maekawa Yuki; Onishi Akishi; Matsushita Keizo; Koide Naoshi; Mandai Michiko; Suzuma Kiyoshi; Kitaoka Takashi; Kuwahara Atsushi; Ozone Chikafumi; Nakano Tokushige; Eiraku Mototsugu; Takahashi Masayo;
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正文语种 eng
中图分类眼科学;
关键词
Culture procedure; neuritogenesis; pluripotent stem cells; retinal ganglion cell; three-dimensional optic vesicle;

机译：培养程序;神经发生;多能干细胞;视网膜神经节细胞;三维视泡;

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1. Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells [J] . Maekawa Yuki, Onishi Akishi, Matsushita Keizo, Current Eye Research . 2016,第4期

机译：优化的培养系统可诱导从小鼠和人类胚胎干细胞分化出来的三维视网膜聚集体中视网膜神经节细胞的神经突生长。
2. Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development, Organization and Neurite Outgrowth from Human Pluripotent Stem Cells [J] . Clarisse M. Fligor, Kirstin B. Langer, Akshayalakshmi Sridhar, Scientific reports. . 2018,第1期

机译：三维视网膜有机体促进从人多能干细胞的视网膜神经节细胞发育，组织和神经突腓术的调查
3. Extracellular matrices preferable to neurite outgrowth from retinal organoids differentiated from mouse embryonic stem cells [J] . Maekawa Yuki, Onishi Akishi, Koide Naoshi, Investigative ophthalmology & visual science . 2016,第12期

机译：从小鼠胚胎干细胞分化的视网膜器有机体的神经沸石过多的细胞外基质
4. Using MEA system in verifying the functionality of retinal pigment epithelium cells differentiated from human embryonic stem cells [C] . N. Nojd, T. Ilmarinen, L. Lehtonen European Conferenc of the International Federation for Medical and Biological Engineering . 2008

机译：使用MEA系统在验证从人胚胎干细胞分化的视网膜颜料上皮细胞的功能
5. An in vitro analysis of biomatrices for CNS tissue repair strategies: Chitosan supports adult neural precursor-derived cell viability, migration, differentiation and retinal explant neurite outgrowth. [D] . Scanga, Vanessa I. 2009

机译：用于中枢神经系统组织修复策略的生物基质的体外分析：壳聚糖支持成人神经前体来源的细胞活力，迁移，分化和视网膜外植体神经突生长。
6. Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development Organization and Neurite Outgrowth from Human Pluripotent Stem Cells [O] . Clarisse M. Fligor, Kirstin B. Langer, Akshayalakshmi Sridhar, -1

机译：三维视网膜类器官有助于人类多能干细胞对视网膜神经节细胞发育组织和神经突生长的研究。
7. Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development, Organization and Neurite Outgrowth from Human Pluripotent Stem Cells [O] . Clarisse M. Fligor, Kirstin B. Langer, Akshayalakshmi Sridhar, 2018

机译：三维视网膜有机体促进从人多能干细胞的视网膜神经节细胞发育，组织和神经突腓术的调查

Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells

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