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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Membrane Tension in Negatively Charged Lipid Bilayers in a Buffer under Osmotic Pressure
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Membrane Tension in Negatively Charged Lipid Bilayers in a Buffer under Osmotic Pressure

机译：在渗透压下缓冲液中带负电荷的脂质双层的膜张力

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Osmotic pressure (Pi) induces membrane tension in cell membranes and the lipid bilayers of vesicles and plays an important role in the functions and physical properties of these membranes. We recently developed a method to determine quantitatively the membrane tension of giant unilamellar vesicles (GUVs) under. and applied it to GUVs comprising electrically neutral dioleoylphosphatidylcholine (DOPC). Here, we examined the effect of. on GUVs composed of DOPC and negatively charged dioleoylphosphatidylglycerol (DOPG) in a buffer containing a physiological concentration of ions. First, we examined the rate constant, k(r), for constant tension (sigma(ex))-induced rupture of DOPG/DOPC (4/6)-GUVs under Pi and obtained the dependence of k(r) on sigma(ex) in GUVs for various values of Pi. Comparing this dependence in the absence of Pi provided values for membrane tension due to Pi, sigma(osm), which agree with the theoretical values within the experimental error. The values of sigma(osm) for DOPG/DOPC-GUVs were smaller than those for DOPC-GUVs under the same Pi. Two factors, that is, the solute concentration in a GUV suspension and the elastic modulus of the GUV membrane, can reasonably explain this difference based on the theory of sigma(osm). We also examined the effect of Pi on the rate constant, k(FF), for the transbilayer movement of lipid molecules in single GUVs. The values of kFF increased with increasing Pi, indicating that k(FF) increased with sigma(osm). This result supports the existence of prepores in stretched lipid bilayers. Based on these results, we discuss the membrane tension of DOPG/DOPC-GUVs under Pi.
机译：渗透压（PI）诱导细胞膜中的膜张力和囊泡的脂质双层，并在这些膜的功能和物理性质中起重要作用。我们最近开发了一种定量测定巨型Unilamellar囊泡（GUV）的膜张力的方法。并将其施加到包含电中性二酰磷脂酰氨基吡啶（DOPC）的GUV。在这里，我们检查了效果。在含有离子的生理浓度的缓冲液中，由DOPC和带负电荷的二脲酰基磷脂酰甘油（DOPG）组成的GUV。首先，我们检查了恒定张力的速率常数K（R） - 恒定张力（Sigma（ex）） - 在Pi下诱导的DOPG / DOPC（4/6）-GUVs破裂并获得K（R）对Sigma的依赖性（ EX）在GUV中进行各种PI值。在缺乏Pi的这种依赖性比较提供了一种用于膜张力由于π，σ（OSM）的值，这与在实验误差之内的理论值一致。用于DOPG / DOPC-GUV的SIGMA（OSM）的值小于同一PI下的DOPC-GUV的值。两种因素，即Guv悬浮液中的溶质浓度和GUV膜的弹性模量，可以合理地根据SIGMA（OSM）的理论来解释这种差异。我们还研究了PI对单一GUV中脂质分子的跨膜运动的速率常数K（FF）的影响。 KFF的值随着PI的增加而增加，表明k（FF）随SIGMA（OSM）而增加。该结果支持在拉伸的脂质双层中存在备忘。基于这些结果，我们讨论了PI下的DOPG / DOPC-GUV的膜张力。

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