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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Critical Temperature of 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine Monolayers and Its Possible Biological Relevance
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Critical Temperature of 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine Monolayers and Its Possible Biological Relevance

机译：1-palmItoyl-2-OXEOYL-SN-甘油-3-磷乙醇胺单层的临界温度及其可能的生物相关性

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Because transmembrane proteins (TMPs) can be obtained with sufficient purity for X-ray diffraction studies more frequently than decades ago, their mechanisms of action may now be elucidated. One of the pending issues is the actual interplay between transmembrane proteins and membrane lipids. There is strong evidence of the involvement of specific lipids with some membrane proteins, such as the potassium crystallographically sited activation channel (KcsA) of Streptomyces lividans and the secondary transporter of lactose LacY of Escherichia coli, the activities of which are associated with the presence of anionic phospholipids such as the phosphatidylglycerol (PG) and phosphatidyethanolamine (PE), respectively. Other proteins such as the large conductance mechanosensitive channel (MscL) of E. coli seem to depend on the adaptation of specific phospholipids to the irregular surface of the integral membrane protein. In this work we investigated the lateral compressibility of two homoacid phosphatidylethanolamines (one with both acyl chains unsaturated (DOPE), the other with the acyl chains saturated (DPPE)) and the heteroacid phosphatidyletanolamine (POPE) and their mixtures with POPG. The liquid expanded (LE) to liquid condensed (LC) transition was observed in POPE at a temperature below its critical temperature (T-c = 36 degrees C). Because Tc lies below the physiological temperature, the occurrence of this phase transition may have something to do with the functioning of LacY. This magnitude is discussed within the context of the experiments carried out at temperatures below the T-c of POPE at which the activity of Lac Y and other TMPs are frequently studied.
机译：因为可以以多于几十年前更频繁地以足够的纯度纯度获得跨膜蛋白（TMPs），所以现在可以阐明它们的作用机制。待决问题之一是跨膜蛋白和膜脂质之间的实际相互作用。有强有力的证据表明特定脂质与一些膜蛋白的参与，例如钾晶型活化通道（KCSA）的链霉菌和乳糖花瓣的二级转运蛋白，其活性与存在的活动有关阴离子磷脂如磷脂酰甘油（PG）和磷脂磷脂（PE）。其他蛋白质如大肠杆菌的大电导机械敏感通道（MSCl）似乎取决于特定磷脂的适应于整体膜蛋白的不规则表面。在这项工作中，我们研究了两种同种酸磷脂酰乙醇胺的横向可压缩性（一种用酰基链不饱和（掺杂），另一种用酰基链饱和（DPPE））和杂酸磷脂酰乙酰胺（POPE）及其与杨树的混合物。在低于其临界温度（T-C = 36℃）的温度下观察到液体膨胀（LC）转变的液体缩合（LC）转变。因为TC低于生理温度，所以这种相变的发生可能与花边的运作有关。在低于POPE的T-C的温度下进行的实验的上下文中讨论了这种幅度，其中经常研究LAC Y和其他TMP的活性。

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