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Cloning, overexpression, purification and characterization of maltooligosyltrehalose synthase from a cyanobacterium, Anabaena 7120

机译:蓝藻鱼腥藻7120的麦芽寡糖基海藻糖合酶的克隆,过表达,纯化和鉴定

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摘要

Trehalose is a non-reducing sugar with How energy (1 kcal mol super(-1)), withstands 100 degree C and is functional in the pH range 3.5-10 (ref. 1). Therefore, it is used as a stabilizer and preservative of foodstuff, cryoprotectant for cells in medicine, in cosmetics and various biotechnological purposes super(2). It has also been reported as a signalling molecule of higher plants super(3-5), and plays an important role in Mycobacterium tuberculosis-host interactions super(6) and in Huntingdon's and Alzheimer's diseases super(7,8). There are five types of trehalose biosynthetic pathways reported in various organisms super(1). One of the pathways having maltooligosyltrehalose synthase (MTSase) (all0167) and maltooligosyltrehalose trehalohydrolase (MTHase) (all0168) enzymes has superiority in trehalose production (up to 80-92%) in many bacteria super(2). A gene cluster constituting trehalose biosynthesizing enzymes, MTSase and MTHase, along with trehalase (all0168), has been reported in Anabaena 7120 (ref. 9) and is maximally expressed in Anabaena 7120 exposed to 150 mM NaCl (refs 10, 11). In addition, increase in trehalose yield may be achieved through manipulation of such enzymes.
机译:海藻糖是一种非还原糖,具有多少能量(1 kcal mol super(-1)),可承受100摄氏度的温度,并在3.5-10的pH范围内起作用(参考文献1)。因此,它被用作食品的稳定剂和防腐剂,医药,化妆品和各种生物技术用途中的细胞冷冻保护剂super(2)。它也被报道为高等植物super(3-5)的信号分子,在结核分枝杆菌-宿主相互作用super(6)以及亨廷登氏和阿尔茨海默氏病super(7,8)中起着重要作用。在各种生物中报告了五种类型的海藻糖生物合成途径super(1)。具有麦芽寡糖基海藻糖合酶(MTSase)(all0167)和麦芽寡糖基海藻糖海藻水解酶(MTHase)(all0168)的途径之一在许多细菌中都具有海藻糖产生优势(高达80-92%)(2)。已经在Anabaena 7120(ref。9)中报道了构成海藻糖生物合成酶MTSase和MTHase与海藻糖酶(all0168)的基因簇,并且在暴露于150 mM NaCl的Anabaena 7120中最大表达了该基因簇(refs 10、11)。另外,可通过操纵此类酶来实现海藻糖产量的增加。

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