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首页> 外文期刊>The journal of physical chemistry, C. Nanomaterials and interfaces >Plasmonic Metal-Insulator-Metal Capped Polymer Nanopillars for SERS Analysis of Protein-Protein Interactions
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Plasmonic Metal-Insulator-Metal Capped Polymer Nanopillars for SERS Analysis of Protein-Protein Interactions

机译:质子算子 - 绝缘体 - 金属封端的聚合物纳米粒子,用于蛋白质 - 蛋白质相互作用的SERS分析

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摘要

The advancement of SERS as an analytical tool requires substrates that provide both sensitive and reproducible measurements. In this work, a gold-titanium dioxide-gold metal-insulator-metal capped polymer nanopillar array SERS substrate is presented and optimized for SERS-based biosensing applications. The optical properties of the multilayered nano antenna array are investigated using a combination of simulation and experimental studies. It is found that hot spot engineering, the plasmon resonance of the array, and cavity structure optimization all contribute to fundamental SERS sensor properties such as enhancement factor and enhancement factor uniformity, which are critically studied using this highly tunable device. A spatially averaged enhancement factor of (2.4 +/- 0.8) X 10(7) with sufficient error for quantitative studies is demonstrated at an excitation wavelength of 633 nm. The label-free detection of protein-protein interactions on the metal-insulator-metal nanopillar array surface is then demonstrated including for the cancer biomarker cancer antigen 125 at a concentration of 100 ng/mL.
机译:SERS的作为分析工具的进步,需要同时提供灵敏的和可再现的测量的衬底。在这项工作中,金 - 二氧化钛 - 金的金属 - 绝缘体 - 金属封端的聚合物纳米柱阵列SERS衬底,提出和用于基于SERS的生物感测应用进行了优化。多层纳米天线阵列的光学属性是使用模拟和实验研究的组合影响。据发现,热点工程,阵列的等离子共振,以及腔结构优化都有助于基本SERS传感器性能如增强因子和增强因子均匀性,这是使用这种高度可调谐设备严重影响。 (2.4 +/- 0.8)×10(7)有足够的误差进行定量研究的空间平均增强因子证明在633nm的激发波长。然后蛋白质 - 蛋白质相互作用的金属 - 绝缘体 - 金属纳米柱阵列表面上的无标记检测证明,包括在100纳克/毫升的浓度的肿瘤标志物癌抗原125。

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