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首页> 外文期刊>Current Eye Research >Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria)
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Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria)

机译：用LDV飞秒激光（Z6）（齐默尔）和一个微角膜刀（莫里亚）对角膜深层切割后的基质床的扫描电子显微镜评价

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Purpose: To compare the stromal bed surface quality and the accuracy of dissection depth after deep lamellar cuts using the Leonardo Da Vinci (LDV) femtosecond laser (Z6) and the ONE Microkeratome.Methods: Deep lamellar cuts were performed on nine human donor corneoscleral buttons: five with the LDV femtosecond (FS) laser (Z6) (Ziemer) and four with the ONE Microkeratome (MK) (Moria). Corneal thickness was measured with ultrasound pachymetry before and after the dissection. The Stromal bed quality was evaluated using light microscopy (n = 4) and scanning electron microscopy (SEM) (n = 9). The surface roughness on SEM images was graded on the scale of 1 (smoothest) to 5 (roughest) by four observers, blinded to the method used. Particle analysis on the SEM images was performed in order to have an objective measure of smoothness.Results: The achieved dissection depth using the FS laser was 496.4 46.4 mu m when attempting 500 mu m and 474 +/- 60 mu m with the microkeratome when attempting 350 mu m. Histological evaluation of the corneoscleral buttons by both light and electron microscopy showed significantly smoother surface using the FS laser compared to the microkeratome. There were fewer and smaller particles observed in the SEM images of FS laser cut buttons (p < 0.001).The average observer based score of anterior surface roughness (50x) was 2.2 for the FS laser and 3.9 for the microkeratome dissections (p < 0.001).Conclusions: The LDV femtosecond laser (Z6) platform is capable of creating deep corneal lamellar dissection with smoother surface quality and with more predictable cut depth as compared to the One Microkeratome.
机译：目的：比较莱昂纳多·达·芬奇（LDV）飞秒激光（Z6）和ONE微型角膜刀在深层状切割后的基质床表面质量和解剖深度的准确性。方法：在9个人类供体角膜巩膜纽扣上进行深层状切割：五个用LDV飞秒（FS）激光（Z6）（Ziemer），四个用一个微角膜刀（MK）（Moria）。在解剖之前和之后，通过超声测厚仪测量角膜厚度。使用光学显微镜（n = 4）和扫描电子显微镜（SEM）（n = 9）评估基质床质量。 SEM图像上的表面粗糙度由四个观察者以1（最平滑）到5（最粗糙）的等级进行分级，而对所使用的方法不了解。结果：在使用FS激光时，当尝试使用500微米的显微镜时，使用FS激光获得的解剖深度为496.4 46.4微米;当使用微型角膜刀时，则达到474 +/- 60微米。尝试350微米通过光学和电子显微镜对角巩膜纽扣的组织学评估显示，与微型角膜刀相比，使用FS激光的表面明显更光滑。 FS激光切割按钮的SEM图像中观察到的颗粒越来越少（p <0.001）.FS激光的平均观察者平均前表面粗糙度（50x）分数是FS激光为2.2，微角膜切开术为3.9（p <0.001） ）结论：与One Microkeratome相比，LDV飞秒激光（Z6）平台能够产生深角膜层状解剖，具有更平滑的表面质量和更可预测的切深。

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