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A 3D‐printed polycaprolactone/β‐tricalcium phosphate mandibular prosthesis: A pilot animal study

机译:一种3D印刷聚己内酯/β-三钙悬崖下颌假体:飞行员动物研究

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Objective In this study, we assessed the effectiveness of a tonsil‐derived mesenchymal stem cell (TMSC)‐transplanted polycaprolactone/beta‐tricalcium phosphate prosthesis (specifically designed for easier fixing and grafting with a single scaffold) on rabbit mandible osteogenesis. Methods The mandibles of 18 rabbits were exposed, and 10?×?8‐mm bone defects were made. Two rabbits did not receive implants; four were reconstructed with the scaffold control (SC) (SC group); four were reconstructed with scaffolds soaked in peripheral blood (PB) (PB group); four were reconstructed with TMSC‐transplanted scaffolds (TMSC group); and four were reconstructed with differentiated osteocyte‐transplanted scaffolds (DOC) (DOC group). Each rabbit was sacrificed 12?weeks after surgery, and the area of new bone formation was investigated by mechanical testing, histology, and micro‐computed tomography. Results More extended and denser new bone masses were observed in the TMSC and DOC groups, although fibrosis and vascular formation levels were similar in all groups, suggesting that the dual‐structured scaffold alone provides a good environment for bone attachment and regeneration. The bone volumes of representative scaffolds from the SC, PB, TMSC, and DOC groups were 43.12, 48.35, 53.10, and 57.44% of the total volumes, respectively. Conclusion The design of the scaffold resulted in effective osteogenesis, and TMSCs showed osteogenic potency, indicating that their combination could enable effective bone regeneration. Level of Evidence NA Laryngoscope , 130:358–366, 2020
机译:目的在这项研究中,我们评估了扁桃体衍生的间充质干细胞(TMSC) - 翻晶的聚己内酯/β-三钙磷酸钙假体(专门设计用于在兔子颌骨骨质发生上进行磷酸钙的假体的有效性。方法采用18只兔子的颌骨,并进行了10?×8mm骨缺陷。两只兔子没有接受植入物;用脚支架控制(SC)重建四个;用浸泡在外周血(PB)(PB组)中重建四个;用TMSC移植的支架(TMSC组)重建四种;用分化的骨细胞移植的支架(DOC组)重建四种,并重建四个。手术后每只兔子处死12周,通过机械测试,组织学和微计算机断层扫描来研究新的骨形成面积。结果在TMSC和DOC组中观察到更加延长和更密集的新骨质量,尽管在所有群体中纤维化和血管形成水平相似,但是单独的双结构支架为骨附着和再生提供了良好的环境。来自SC,Pb,TMSC和DOC组的代表性支架的骨体积分别为43.12,48.35,53.10和总量的57.44%。结论支架的设计导致有效骨质发生,TMSCs显示出骨质发生效力,表明它们的组合能够实现有效的骨再生。证据水平na喉镜,130:358-366,2020

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