...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Experimental Biology >Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect
【24h】

Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect

机译：快速冷硬化可防止南极昆虫中的亚致死冻伤

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rapid cold hardening (RCH) is a type of beneficial phenotypic plasticity that occurs on extremely short time scales (minutes to hours) to enhance insects' ability to cope with cold snaps and diurnal temperature fluctuations. RCH has a well-established role in extending lower lethal limits, but its ability to prevent sublethal cold injury has received less attention. The Antarctic midge, Belgica antarctica, is Antarctica's only endemic insect and has a well-studied RCH response that extends freeze tolerance in laboratory conditions. However, the discriminating temperatures used in previous studies of RCH are far below those ever experienced in the field. Here, we tested the hypothesis that RCH protects against non-lethal freezing injury. Larvae of B. antarctica were exposed to control (2 degrees C), direct freezing (-9 degrees C for 24 h) or RCH (-5 degrees C for 2 h followed by -9 degrees C for 24 h). All larvae survived both freezing treatments, but RCH larvae recovered more quickly from freezing stress and had a significantly higher metabolic rate during recovery. RCH larvae also sustained less damage to fat body and midgut tissue and had lower expression of two heat shock protein transcripts (hsp60 and hsp90), which is consistent with RCH protecting against protein denaturation. The protection afforded by RCH resulted in energy savings; directly frozen larvae experienced a significant depletion in glycogen energy stores that was not observed in RCH larvae. Together, these results provide strong evidence that RCH protects against a variety of sublethal freezing injuries and allows insects to rapidly fine-tune their performance in thermally variable environments.
机译：快速冷硬化（RCH）是一种有益表型可塑性，发生在极短的时间尺度（分钟到小时）上，以增强昆虫应对冷捕捉和昼夜温度波动的能力。 RCH在延长致命致命局限性方面具有良好的作用，但其防止亚致死伤害的能力受到不太关注。南极地中的MIDGE，比利卡南极洲是南极洲唯一的特有昆虫，并且具有良好的RCH反应，在实验室条件下延伸了冻结耐受性。然而，在RCH的先前研究中使用的鉴别温度远远低于现场经历的那些。在这里，我们测试了RCH保护非致死冷冻损伤的假设。 B.南极洲的幼虫暴露于对照（2℃），直接冷冻（-9℃24小时）或RCH（-5℃，2小时，然后24小时接下来为-9℃）。所有幼虫均在冷冻治疗中存活，但RCH幼虫从冷冻应激中恢复更快，并且在恢复过程中具有显着提高的代谢率。 RCH幼虫对脂肪体和中小型组织造成的损害较少，并具有两种热休克蛋白转录物（HSP60和HSP90）的表达，这与RCH保护免受蛋白质变性一致。 RCH提供的保护导致节能;直接冷冻幼虫在RCH幼虫未观察到的糖原能量储存中经历了显着的枯萎。这些结果在一起提供了强有力的证据，即RCH保护各种止痛性损伤，并允许昆虫在热可变环境中快速微调它们的性能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号