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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Human immunodeficiency virus 1 dual‐target nucleic acid technology improves blood safety: 5 years of experience of the German Red Cross blood donor service Baden‐Württemberg–Hessen
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Human immunodeficiency virus 1 dual‐target nucleic acid technology improves blood safety: 5 years of experience of the German Red Cross blood donor service Baden‐Württemberg–Hessen

机译：人类免疫缺陷病毒1双靶核酸技术可提高血液安全：5年的德国红十字会献血服务Baden-Württemberg-hessen

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摘要

BACKGROUND RNA viruses are associated with a high frequency of mutations because of the missing proofreading function of polymerases, such as reverse transcriptase. Between 2007 and 2010, six blood donations with false‐negative nucleic acid technology (NAT) results were reported in Germany. Therefore, NAT screening in two viral genome regions was introduced by our blood donation service in 2010 on a voluntary basis and became mandatory in Germany since the beginning of 2015. STUDY DESIGN AND METHODS Blood donor screening was done using, in parallel, the German Red Cross (GRC) HIV‐1 CE long terminate repeats (LTR) PCR kit and the GRC HIV‐1 gag CE PCR kit. In total, 7 million blood donations were screened during the study period from 2010 to 2014 with the GRC dual‐target human immunodeficiency virus 1 (HIV‐1) NAT system. Additionally, three suspicious specimens were analyzed by four monotargeted NAT assays and by five dual‐target NAT assays. RESULTS Three of 7 million donations tested negative using the 5’LTR–polymerase chain reaction, but they were positive if amplification was performed in the gag region. HIV antibodies were detected in all three donations. Nucleic acid sequence analysis identified a deletion of 22 bases within the 5’LTR probe binding region. Three different ltr ‐based monotargeted assays missed two donations, except for a low‐reactive result obtained by one of the assays. In total, the detection rates for HIV‐1–positive donations were 37.5% (3/8) for monotargeted assays and 100% (10/10) for dual‐target assays. CONCLUSION The current data demonstrate that dual‐target NAT systems reduce the risk of false‐negative HIV‐1 NAT screening results.
机译：由于缺少聚合酶的缺失功能，例如逆转录酶，因此RNA病毒与高频率的突变有关。在2007年至2010年期间，德国报道了六种具有假阴性核酸技术（NAT）结果的血液捐赠。因此，2010年我们的献血服务在2010年的血液捐赠服务中引入了两种病毒基因组区域的NAT筛选，自2015年初以来，德国的强制性。研究设计和方法血液供血筛选使用，并行，德国红色十字（GRC）HIV-1 CE长终止重复（LTR）PCR试剂盒和GRC HIV-1 GAG CE PCR试剂盒。在2010年至2014年，GRC双靶人免疫缺陷病毒1（HIV-1）NAT系统的研究期间，筛选了700万献血。另外，通过四种单调的NAT测定和五种双靶NAT测定分析了三种可疑标本。结果3700万捐赠3700万次使用5'LTR-聚合酶链反应测试阴性，但如果在GAG区域进行扩增，它们是阳性的。在所有三种捐赠中检测到HIV抗体。核酸序列分析鉴定了5'LTR探针结合区域内的22个碱基的缺失。除了通过其中一个测定获得的低反应性结果，遗传了三种不同的LTR基于两种捐赠的单调分析。总共，用于单调测定的HIV-1阳性捐赠的检测率为37.5％（3/8），用于双靶测定的100％（10/10）。结论目前的数据表明，双重目标NAT系统降低了假阴性HIV-1 NAT筛选结果的风险。

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    Hourfar Kai; Eberle Josef; Müller Markus; Micha Nübling C; Chudy Michael; Kress Julia; Gürtler Lutz; Mayr‐Wohlfart Uschi; Schrezenmeier Hubert; Hellmann Inke; Luhm Jürgen; Kraas Sabine; Ringwald Jürgen; Gubbe Knut; Frank Kerstin; Karl Andreas; Tonn Torsten; Jaeger Maike; Sireis Walid; Seifried Erhard; Schmidt Michael;

  • 作者单位

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

    Virology National Reference Center for Retroviruses Faculty of MedicineMax von Pettenkofer;

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

    Paul‐Ehrlich‐InstitutLangen Germany;

    Paul‐Ehrlich‐InstitutLangen Germany;

    Paul‐Ehrlich‐InstitutLangen Germany;

    Virology National Reference Center for Retroviruses Faculty of MedicineMax von Pettenkofer;

    Institute for Clinical Transfusion Medicine and Immunogenetics UlmUlm Germany;

    Institute for Clinical Transfusion Medicine and Immunogenetics UlmUlm Germany;

    German Red Cross Blood Donor Service North‐East Institute LütjenseeLütjensee Germany;

    German Red Cross Blood Donor Service North‐East Institute LütjenseeLütjensee Germany;

    German Red Cross Blood Donor Service North‐East Institute LütjenseeLütjensee Germany;

    German Red Cross Blood Donor Service North‐East Institute LütjenseeLütjensee Germany;

    German Red Cross Blood Donor Service North‐East Institute PlauenPlauen Germany;

    German Red Cross Blood Donor Service North‐East Institute PlauenPlauen Germany;

    German Red Cross Blood Donor Service North‐East Institute PlauenPlauen Germany;

    German Red Cross Blood Donor Service North‐East Institute DresdenDresden Germany;

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

    German Red Cross Blood Donor Service Baden‐Württemberg–HessenInstitute for Transfusion Medicine and;

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