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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Quality evaluation of frozen apheresis red blood cell storage with 21-day postthaw storage in additive solution 3 and saline-adenine-glucose-mannitol: biochemical and chromium-51 recovery measures.
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Quality evaluation of frozen apheresis red blood cell storage with 21-day postthaw storage in additive solution 3 and saline-adenine-glucose-mannitol: biochemical and chromium-51 recovery measures.

机译：添加到阅览室阅读软件下，将冷冻溶液储存21天的后三烷基储存和盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇：生物化学和铬-51恢复措施评价及盐水 - 葡萄糖 - 甘露醇的质量评价

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BACKGROUND: The Czech Republic decided to build the frozen red blood cell (RBC) bank for military contingency and civil emergency preparedness. Storage methods were validated with (51)Cr recovery measures out to 21 days postthaw. STUDY DESIGN AND METHODS: A total of 139 apheresis RBC units collected in additive solution (additive solution 3 [AS-3] or saline-adenine-glucose-mannitol [SAG-M]) were obtained from volunteers by double erythrocytapheresis; all were sterilely glycerolized, frozen, stored for at least 30 days at a minimum of -65 degrees C in collection or cryogenic bags, thawed, sterilely deglycerolized, and stored at 4 +/- 2 degrees C for up to 21 days in AS-3 or SAG-M. Units were sampled before storage and after thaw weekly. Metabolic intermediates and (51)Cr recovery measures were obtained to determine the index of therapeutic effectiveness (ITE). RESULTS: Despite losses associated with cryoconservation and eventual transfer to cryogenic bags, 3-week storage with optimum ITE was obtained with frozen storage in primary collection or cryogenic bags and postthaw storage in AS-3. Such cells would pass as acceptable in vitro hematology and biochemistry variables. CONCLUSIONS: A system for frozen RBC storage with 3-week, postthaw, liquid storage has been validated. With commercially available components and automated processing, it is capable of supporting civilian rare blood and emergency and international military combat and peacekeeping support use patterns.
机译：背景：捷克共和国决定建立冷冻红细胞（RBC）银行为军事应急和民用应急准备。将储存方法验证（51）CR恢复措施，后21天后。研究设计和方法：通过双红细胞膜从志愿者获得添加剂溶液中收集的139个吸收性RBC单元（添加剂3 [AS-3]或盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇[SAG-M]）;全部对糖化剂，冷冻，在收集或低温袋中至少储存至少30天，储存至少30天，解冻，无菌脱甘油，并在4 +/- 2℃下储存长达21天 - 3或sag-m。单位在储存前和每周冻土后进行采样。获得代谢中间体和（51）CR回收措施，以确定治疗效果指数（ITE）。结果：尽管与低温袋相关的损失，但最终转移到低温袋，在主要收集或低温袋中的冷冻储存和AS-3中的后三烷储存获得3周的储存。这种细胞将通过体外血液学和生物化学变量可接受。结论：经过验证了3周，后三锯的冷冻RBC储存系统，验证了液体储存。随着市售的部件和自动化加工，能够支持平民稀有血液和紧急和国际军事战斗和维持和平支持使用模式。

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