UNIT 27.3 Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae

Bessie W. Kebaara; Kristian E. Baker; Krista D. Patefield; Audrey L. Atkin

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UNIT 27.3 Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae

机译：UNIT 27.3酿酒酵母中无意义介导的mRNA衰变分析

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Nonsense-mediated mRNA decay is a highly conserved pathway that degrades mRNAs with premature termination codons. These mRNAs include mRNAs transcribed from nonsense or frameshift alleles as well as wild-type mRNA with signals that direct ribosomes toterminate prematurely. This unit describes techniques to monitor steady-state mRNA levels, decay rates, and structural features of mRNAs targeted by this pathway, as well as in vivo analysis of nonsense suppression and allosuppression in the yeast Saccharomyces cerevisiae. Protocols for the structural features of mRNA include analysis of cap status, 5′ and 3′ untranslated region (UTR) lengths, and poly(A) tail length. Curr. Protoc.

机译：无意义介导的mRNA衰变是一个高度保守的途径，可降解具有过早终止密码子的mRNA。这些mRNA包括从无义或移码等位基因转录的mRNA，以及带有指导核糖体过早终止的信号的野生型mRNA。该单元描述了监测稳态mRNA水平，衰减率和该途径靶向的mRNA结构特征的技术，以及在酵母中对无意义抑制和同种异体抑制的体内分析。 mRNA的结构特征的协议包括帽状态，5'和3'非翻译区（UTR）长度和poly（A）尾巴长度的分析。 Curr。协议。

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《Current Protocols in Bioinformatics》 |2016年第54期|共27页
作者
Bessie W. Kebaara; Kristian E. Baker; Krista D. Patefield; Audrey L. Atkin;
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正文语种 eng
中图分类遗传学;
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steady-state mRNA level; mRNA decay rate; untranslated region length; 5′-cap; poly(A) tail length; nonsense suppression; allosuppression;

机译：稳态mRNA水平;mRNA衰减率;非翻译区长度;5'-帽;poly（A）尾巴长度;无意义的抑制;失语抑制;

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1. UNIT 27.3 Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae [J] . Bessie W. Kebaara, Kristian E. Baker, Krista D. Patefield, Current Protocols in Bioinformatics . 2016,第Suppla54期

机译：UNIT 27.3酿酒酵母中无意义介导的mRNA衰变分析
2. Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay [J] . Obenoskey Jesseeca, Lane Dakota R., Atkin Audrey L., Frontiers in Molecular Biosciences . 2014,第4期

机译：酿酒酵母SSY5 mRNA对无意义介导的mRNA衰变的免疫力
3. Nonsense-mediated mRNA decay of the ferric and cupric reductase mRNAs FRE1 and FRE2 in Saccharomyces cerevisiae [J] . Peccarelli Megan, Scott Taylor D., Kebaara Bessie W. FEBS letters. . 2019,第22期

机译：废典型介导的致铬和铜克里斯杂志的mRNA腐烂和弗雷2酿酒酵母酿酒酶
4. Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes [C] . Richard E. Green, Benjamin P. Lewis, R. Tyler Hillman, International Conference on Intelligent Systems for Molecular biology . 2003

机译：普遍预测的无意义介导的mRNA衰减的人正常和疾病基因的替代剪接转录物
5. No Nonsense: The Protection of Wild-Type mRNAs From Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae. [D] . Patefield, Krista D. 2016

机译：没有废话：酿酒酵母中的无意义介导的mRNA衰变对野生型mRNA的保护。
6. Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay [O] . Jesseeca Obenoskey, Dakota R. Lane, Audrey L. Atkin, 2014

机译：酿酒酵母SSY5 mRNA对无意义介导的mRNA衰变的免疫力
7. Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay. [O] . Bessie Wanja Kebaara, Jesseeca eObenoskey, Dakota R Lane, 2014

机译：酿酒酵母ssY5 mRNa对无义介导的mRNa衰变的免疫。

UNIT 27.3 Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae

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