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首页> 外文期刊>Vaccine >Attenuated live infectious bronchitis virus QX vaccine disseminates slowly to target organs distant from the site of inoculation
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Attenuated live infectious bronchitis virus QX vaccine disseminates slowly to target organs distant from the site of inoculation

机译：减毒的现场感染性支气管炎病毒QX疫苗缓慢地传播到靶器官远离接种位点的靶器官

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Infectious bronchitis (IB) is a highly contagious respiratory disease of poultry, caused by the avian coronavirus infectious bronchitis virus (IBV). Currently, one of the most relevant genotypes circulating worldwide is IBV-QX (GI-19), for which vaccines have been developed by passaging virulent QX strains in embryonated chicken eggs. Here we explored the attenuated phenotype of a commercially available QX live vaccine, IB Primo QX, in specific pathogens free broilers. At hatch, birds were inoculated with QX vaccine or its virulent progenitor IBV-D388, and postmortem swabs and tissues were collected each day up to eight days post infection to assess viral replication and morphological changes. In the trachea, viral RNA replication and protein expression were comparable in both groups. Both viruses induced morphologically comparable lesions in the trachea, albeit with a short delay in the vaccinated birds. In contrast, in the kidney, QX vaccine viral RNA was nearly absent, which coincided with the lack of any morphological changes in this organ. This was in contrast to high viral RNA titers and abundant lesions in the kidney after IBV D388 infection. Furthermore, QX vaccine showed reduced ability to reach and replicate in conjunctivae and intestines including cloaca, resulting in significantly lower titers and delayed protein expression, respectively. Nephropathogenic IBVs might reach the kidney also via an ascending route from the cloaca, based on our observation that viral RNA was detected in the cloaca one day before detection in the kidney. In the kidney distal tubular segments, collecting ducts and ureter were positive for viral antigen. Taken together, the attenuated phenotype of QX vaccine seems to rely on slower dissemination and lower replication in target tissues other than the site of inoculation. (C) 2019 The Author(s). Published by Elsevier Ltd.
机译：感染性支气管炎（IB）是由禽冠状病毒感染性支气管炎病毒（IBV）引起的家禽呼吸道疾病。目前，全球循环的最相关的基因型之一是IBV-QX（GI-19），其通过在胚胎鸡蛋中传递毒力QX菌株来开发疫苗。在这里，我们探讨了特定病原体的可商购QX活疫苗IB Primo QX的衰减表型。在孵化中，用QX疫苗或其毒性祖先IBV-D388接种鸟类，并且每天收集后效率的拭子和组织，最多八天的感染后患者评估病毒复制和形态学变化。在气管中，病毒RNA复制和蛋白质表达在两个基团中相当。两种病毒在气管中诱导形态学上的类似病变，尽管疫苗的鸟类中具有短暂的延迟。相反，在肾脏中，QX疫苗病毒RNA几乎不存在，这与该器官缺乏缺乏任何形态变化。与IBV D388感染后肾脏中的高病毒RNA滴度和丰富的病变相反。此外，QX疫苗表明，在包括Choaca的结膜和肠道中达到和复制的能力，导致显着降低的滴度和延迟蛋白表达。基于我们观察到在肾脏检测前一天，肾病疗法IBV也可以通过从Cloaca从Cloaca升序到达肾脏来达到肾脏。在肾脏远端管状段中，收集管道和输尿管为病毒抗原阳性。总之，QX疫苗减毒表型似乎依赖于比接种部位等靶组织传播速度较慢和较低的复制。 （c）2019年作者。 elsevier有限公司出版

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