Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule

Fry Lindsay M.; Bastos Reginaldo G.; Stone Brad C.; Williams Laura B.; Knowles Donald P.; Murphy Sean C.

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Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule

机译：基因枪DNA免疫牛诱导体液和CD4 T细胞介导的免疫应答，对细胞膜帕尔氏菌多态性免疫肿瘤分子

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Theileria parva kills over one million cattle annually in sub-Saharan Africa. Parasite genetic complexity, cellular response immunodominance, and bovine MHC diversity have precluded traditional vaccine development. One potential solution is gene gun (GG) immunization, which enables simultaneous administration of one or more DNA-encoded antigens. Although promising in murine, porcine, and human vaccination trials, bovine GG immunization studies are limited. We utilized the model T. parva antigen, polymorphic immunodominant molecule (PIM) to test bovine GG immunization. GG immunization using a mammalian codon optimized PIM sequence elicited significant anti-PIM antibody and cell mediated responses in 7/8 steers, but there was no difference between immunized and control animals following T. parva challenge. The results suggest immunization with PIM, as delivered here, is insufficient to protect cattle from T. parva. Nonetheless, the robust immune responses elicited against this model antigen suggest GG immunization is a promising vaccine platform for T. parva and other bovine pathogens. Published by Elsevier Ltd.

机译：在撒哈拉以南非洲，Theileria Parva每年杀死超过一百万牛。寄生虫遗传复杂性，细胞反应免疫肿瘤和牛MHC多样性排除了传统疫苗的发育。一种潜在的解决方案是基因枪（GG）免疫，这使得能够同时施用一种或多种DNA编码的抗原。虽然小鼠，猪和人类疫苗接种试验有前途，但牛GG免疫研究有限。我们利用型T.Parva抗原，多态免疫肿瘤分子（PIM）来试验牛GG免疫。使用哺乳动物密码子的GG免疫优化PIM序列引发了7/8位的显着的抗PIM抗体和细胞介导的反应，但在T.Parva挑战之后免疫和对照动物之间没有差异。结果表明，这里递送的PIM免疫，不足以保护来自T. Parva的牛。尽管如此，对该模型抗原引发的鲁棒免疫应答表明GG免疫是T.Parva和其他牛病原体的有望疫苗平台。 elsevier有限公司出版

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《Vaccine》 |2019年第12期|共8页
作者
Fry Lindsay M.; Bastos Reginaldo G.; Stone Brad C.; Williams Laura B.; Knowles Donald P.; Murphy Sean C.;
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作者单位

ARS Anim Dis Res Unit USDA POB 646630 Pullman WA 99164 USA;

Washington State Univ Dept Vet Microbiol &

Pathol POB 647040 Pullman WA 99164 USA;

Univ Washington Dept Lab Med Med Ctr 1959 NE Pacific St Seattle WA 98195 USA;

Washington State Univ Dept Vet Microbiol &

Pathol POB 647040 Pullman WA 99164 USA;

Washington State Univ Dept Vet Microbiol &

Pathol POB 647040 Pullman WA 99164 USA;

Univ Washington Dept Lab Med Med Ctr 1959 NE Pacific St Seattle WA 98195 USA;

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原文格式 PDF
正文语种 eng
中图分类医学免疫学;
关键词
DNA vaccination; East Coast Fever; Gene gun immunization; Cattle; Vaccine;

机译：DNA疫苗接种;东海岸发烧;基因枪免疫;牛;疫苗;

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1. Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule [J] . Fry Lindsay M., Bastos Reginaldo G., Stone Brad C., Vaccine . 2019,第12期

机译：基因枪DNA免疫牛诱导体液和CD4 T细胞介导的免疫应答，对细胞膜帕尔氏菌多态性免疫肿瘤分子
2. Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-I[sup3 responses but not CTL responses [J] . Mwangi Duncan M, Honda Yoshikazu, Graham Simon P, Veterinary Immunology and Immunopathology . 2011,第3a4期

机译：在用Theileria parva候选疫苗抗原免疫之前，用DNA编码的Flt3L和GM-CSF处理牛会诱导CD4和CD8 T细胞IFN-I [sup3反应，但不诱导CTL反应
3. Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva [J] . Graham SP, Saya R, Awino E, Veterinary Immunology and Immunopathology . 2007,第3a4期

机译：免疫刺激性CpG寡脱氧核苷酸增强了牛对原生动物寄生虫Theileria parva多态免疫优势分子的CD4 +细胞毒性T淋巴细胞反应的诱导。
4. INTRADERMAL ADMINISTRATION OF SYNTHETIC DNA VACCINES INDUCE ROBUST CELLULAR AND HUMORAL IMMUNE RESPONSES [C] . Jean D. Boyer Conference on vaccine technology VI . 2018

机译：皮内管理合成DNA疫苗诱导强健的细胞和体液免疫反应
5. Suppression of the T-cell dependent humoral immune response by Delta(9)-tetrahydrocannanbinol involves inpairment of CD40-CD40 ligand interaction. [D] . Ngaotepprutaram, Thitirat. 2013

机译：Delta（9）-四氢大麻酚抑制T细胞依赖性体液免疫应答涉及CD40-CD40配体相互作用的损害。
6. Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule [O] . Lindsay M. Fry, Reginaldo G. Bastos, Brad C. Stone, -1

机译：牛的基因枪DNA免疫诱导体液和CD4 T细胞介导的针对泰勒虫小虫多态性免疫优势分子的免疫反应
7. Analyses of genes encoding Theileria parva p104 and polymorphic immunodominant molecule (PIM) reveal evidence of the presence of cattle-type alleles in the South African T. parva population [O] . Sibeko, K. P., Collins, N. E., Oosthuizen, M. C., 2011

机译：编码Theleria parva p104和多态性免疫优势分子（PIM）的基因分析揭示了南非T. parva种群中存在牛型等位基因的证据

Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule

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