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Evaluation of the protective effects of a nanogel-based vaccine against rabbit hepatitis E virus

机译：纳米凝胶基疫苗对兔乙型肝炎病毒的保护作用评价

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摘要

Infection with hepatitis E virus (HEV) has raised serious public health concerns worldwide. In this study, a nanogel-based vaccine encapsulating the capsid protein of rabbit HEV was developed and its protective efficacy was compared with a subunit vaccine. A total of 23 rabbits were divided into 5 groups: (1) negative control (n = 4), (2) positive control (n = 4), (3) nanogel control (n = 5), (4) nanogel vaccine (n = 5), and (5) subunit vaccine (n = 5). Rabbits were vaccinated two times, at weeks 0 and 1, with nanogel and subunit vaccines, respectively, and challenged with rabbit HEV at week 4. By week 11, rabbits vaccinated with the nanogel vaccine produced higher antibodies than those vaccinated with the subunit vaccine. Fecal viral shedding and viremia were identified in rabbits of the positive and nanogel control groups at weeks 6-10. However, there was no viral shedding and viremia in rabbits immunized with both the nanogel and subunit vaccines. Alanine aminotransferase and aspartate aminotransferase levels were not elevated in any rabbit. However, histopathological examination revealed much less hepatic inflammation in rabbits of the nanogel vaccine group compared to the positive and nanogel control groups. Significant increases in IL-12 and IFN-gamma levels were identified from rabbits immunized with the nanogel vaccine. Collectively, these results indicate that the newly developed nanogel vaccine induced sufficient immunity leading to complete protection from HEV infection in rabbits. Application of this vaccine should be considered as a preventive measure against HEV infection in other animal species and humans. (C) 2019 Elsevier Ltd. All rights reserved.
机译：用乙型肝炎感染病毒（HEV）在全球范围内提高了严重的公共卫生问题。在该研究中，开发了一种纳米凝胶的疫苗，其封装了兔HEV的辣椒蛋白，并将其保护效果与亚基疫苗进行了比较。将共23只兔子分为5组：（1）阴性对照（n = 4），（2）阳性对照（n = 4），（3）纳米凝胶对照（n = 5），（4）纳米凝胶疫苗（ n = 5），和（5）亚基疫苗（n = 5）。兔子在第0周和1周内接种兔子，分别与纳孔和亚基疫苗接种，并在第4周与兔HEV攻击。在第11周，用纳米凝胶疫苗接种的兔子产生高于与亚基疫苗接种的抗体的兔子。在6-10周内以阳性和纳米凝胶对照组的兔鉴定粪便病毒脱落和病毒血症。然而，没有用纳米凝胶和亚基疫苗免疫的兔中没有病毒脱落和病毒血症。丙氨酸氨基转移酶和天冬氨酸氨基转移酶水平未在任何兔子中升高。然而，与阳性和纳米凝胶对照组相比，组织病理学检查揭示了纳米凝胶疫苗基团的兔子中的肝脏炎症。用纳米凝胶疫苗免疫的兔鉴定IL-12和IFN-Gamma水平的显着增加。总的来说，这些结果表明，新开发的纳米凝胶疫苗诱导了足够的免疫力，导致兔子中的HEV感染完全保护。该疫苗的应用应被视为对其他动物物种和人类中HEV感染的预防措施。 （c）2019 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Vaccine》 |2019年第40期|共7页
  • 作者

    Park Byung-Joo; Ahn Hee-Seop; Han Sang-Hoon; Go Hyeon-Jeong; Lee Joong-Bok; Park Seung-Yong; Song Chang-Seon; Lee Sang-Won; Paik Hyun-Jong; Choi Yang-Kyu; Choi In-Soo;

  • 作者单位

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

    Pusan Natl Univ Dept Polymer Sci &

    Engn San 30 Jangjeon 2 Dong Busan 609735 South Korea;

    Konkuk Univ Dept Lab Anim Med Coll Vet Med 120 Neungdong Ro Seoul 05029 South Korea;

    Konkuk Univ Coll Vet Med Dept Infect Dis 120 Neungdong Ro Seoul 05029 South Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    Rabbit; HEV; Nanogel; Subunit; Vaccine;

    机译：兔子;HEV;纳孔;亚基;疫苗;

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