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首页> 外文期刊>Vaccine >Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases
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Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

机译：Fowl Adenovirus Serotype 4的Penton-Dodecahedron作为控制相关疾病的疫苗候选者

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In some serotypes of adenovirus (Ad), the penton base and attached trimeric fiber assemble into dodecameric virus-like particles called penton-dodecahedron (Pt-Dd), which can be internalized and used to deliver the vaccine antigens and drugs. Fowl adenovirus serotype 4 (FAdV-4) is an important pathogen, causing seriously economic loss to poultry industry in China and other counties. Pt-Dd particles from FAdV-4 infected cells, as well as in those infected with recombinant human Ad expressing fiber-1, fiber-2, and penton base of FAdV-4, were visualized by transmission electron microscopy. For the first time, we proved that FAdV-4 produced Pt-Dd in infected cells. Pt-Dd can also be assembled by the over expressed recombinant proteins fiber-1, fiber-2, and penton base. Pt-Dd, as well as the recombinant proteins fiber-1, fiber-2, and penton base, were then used to immunize chickens. The humoral immune response, expression of selected immune molecules and challenge results were used to evaluate the immune efficacy of the vaccine candidates. Pt-Dd induced the highest level of enzyme-linked immunosorbent assay antibodies and significant high levels (p & 0.05) of interferon gamma, interleukin-4, and major histocompatibility complex II expression in peripheral blood mononuclear cells at 48 h post-infection. The challenge results showed that Pt-Dd, inactivated FAdV-4 vaccine, and fiber-1 induced the best protection (100%), followed by fiber-2 (80%) and penton base (67%). The present study showed that FAdV-4-Pt-Dd and recombinant fiber-1 are promising FAdV-4 vaccine candidates and could be used to replace the tissue-sourced inactivated FAdV-4 vaccine. (C) 2018 Elsevier Ltd. All rights reserved.
机译：在腺病毒（Ad）的一些血清型中，柱碱和附着的三聚体纤维组装成称为柱状二十甲醚（PT-DD）的十二聚酰胺病毒样颗粒，其可以内化并用于递送疫苗抗原和药物。 Fowl腺病毒血清型4（Fadf-4）是一个重要的病原体，导致中国和其他县的家禽产业严重经济损失。通过透射电子显微镜通过透射电子显微镜可视化来自Fadom-4感染细胞的Pt-DD颗粒，以及感染的重组人Ad，纤维-1，纤维-2和恒定柱。我们首次证明了在感染细胞中产生的PT-DD产生PT-DD。 PT-DD也可以通过过度表达的重组蛋白纤维-1，纤维-2和柱碱组装。然后使用PT-DD，以及重组蛋白纤维-1，纤维-2和恒顿碱，用于免疫鸡。液体免疫应答，选择的免疫分子和攻击结果的表达用于评估疫苗候选物的免疫效果。 PT-DD诱导最高水平的酶联免疫吸附测定抗体和显着的高水平（P＆amp; 0.05）的干扰素γ，白细胞介素-4和在48小时后的外周血单核细胞中的主要组织相容性复合物II表达。感染。挑战结果表明，PT-DD，灭活的排名疫苗和纤维-1诱导最佳保护（100％），然后是纤维-2（80％）和恒顿碱（67％）。目前的研究表明，Fadv-4-Pt-DD和重组纤维-1是有前途的疫苗候选者，可用于取代组织源性灭活的富差4疫苗。 （c）2018年elestvier有限公司保留所有权利。

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