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The EB66 (R) cell line as a valuable cell substrate for MVA-based vaccines production

机译:EB66(R)细胞系作为基于MVA的疫苗生产的有价值的细胞基底

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The selection of a cell substrate is a critical step for the development and manufacturing of a viral vaccine candidate. Several parameters such as cell susceptibility and permissiveness to the viral pathogens but also performance in terms of viral antigens quality and production yields are important considerations when identifying the ideal match between a viral vaccine and cell substrate. The modified vaccinia virus Ankara (MVA) is a replication-deficient viral vector that holds great promise as a vaccine platform, however only limited cell substrates have been tested or are available for industrialization. Here we evaluate the duck embryo-derived EB66 (R) cell line as potential cell substrate for MVA production. To this end, we used two recombinant MVA constructs and demonstrated that EB66 (R) cells are propagating the tested MVA viruses very efficiently, while preserving viral attenuation and transgene expression for up to 20 serial passages. Furthermore we developed upstream and downstream processes that enable industrialization of the virus production. In conclusion, we showed that EB66 (R) cells can be used as potent cell substrate for MVA-based vaccines and represent therefore an attractive alternative for vaccine production. (C) 2016 The Authors. Published by Elsevier Ltd.
机译:细胞基底的选择是用于显影和制造病毒疫苗候选的关键步骤。诸如细胞敏感性和允许的病毒病原体的几种参数,但在病毒抗原质量和产量方面的性能是在识别病毒疫苗和细胞基底之间的理想匹配时是重要的考虑因素。改性痘苗病毒Ankara(MVA)是一种复制缺陷的病毒载体,其作为疫苗平台具有很大的希望,但只有有限的细胞基板已经过测试或可用于工业化。在这里,我们评估鸭胚衍生的EB66(R)细胞系作为MVA生产的潜在细胞基底。为此,我们使用了两种重组MVA构建体,并证明了EB66(R)细胞非常有效地繁殖测试的MVA病毒,同时保持病毒衰减和转基因表达至多20个序列通道。此外,我们开发了能够生产病毒生产的上游和下游流程。总之,我们表明EB66(R)细胞可以用作基于MVA的疫苗的有效细胞基质,因此因此表示疫苗生产的有吸引力的替代方案。 (c)2016年作者。 elsevier有限公司出版

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