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首页> 外文期刊>Vaccine >A bivalent vaccine derived from attenuated Salmonella expressing O-antigen polysaccharide provides protection against avian pathogenic Escherichia coli O1 and O2 infection
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A bivalent vaccine derived from attenuated Salmonella expressing O-antigen polysaccharide provides protection against avian pathogenic Escherichia coli O1 and O2 infection

机译：源自表达O-抗原多糖的减毒沙门氏菌的二价疫苗提供针对禽病原大肠杆菌O1和O2感染的保护

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摘要

Avian pathogenic Escherichia coli (APEC), a leading cause of avian airsacculitis and colibacillosis, is responsible for significant economic loss in the poultry industry. APEC serogroups O1, O2, and O78 are predominantly associated with disease. Lipopolysaccharide (LPS) O-antigen has been shown to be a potent antigen for inducing specific protective immune responses. Therefore, we sought to develop a multivalent polysaccharide vaccine to prevent most APEC infections. We previously reported the stable expression of plasmid pSS27 encoding the APEC O1 O-antigen gene cluster (10.8 kb) in attenuated Salmonella enterica serovar Typhimurium S740 provided excellent protection against APEC O1 challenge. In this study, the plasmid pSS28 harboring the APEC O2 O-antigen polysaccharide gene cluster (15.5 kb) was constructed. Biosynthesis of pSS28-encoded APEC O2 O-antigen in Salmonella vaccine strain S740 was validated by Western blot. The recombinant Salmonella vaccine strain S740 (pSS28) elicited homologous protection against virulent wild-type APEC O2 challenge in a chicken model. Furthermore, through equal-volume mixing the two monovalent vaccine strains 5740 (pSS27) and S740 (pSS28), a bivalent vaccine candidate against both APEC O1 and O2 was developed. Immunization of chickens with the bivalent vaccine elicited production of serum IgG and mucosal sIgA antibodies against the LPS of both APEC O1 and O2. Moreover, antibodies induced by the bivalent vaccine promoted opsonization, provoked complement-mediated bactericidal activity, and elicited protection against lethal challenge with both virulent APEC O1 and O2 strains. These results demonstrate that the bivalent vaccine comprised of 5740 (pSS27) and 5740 (pSS28) is a promising vaccine candidate against APEC O1 and O2 infection. (C) 2018 Elsevier Ltd. All rights reserved.
机译：禽致病性大肠杆菌（APEC）是禽空气炎和加利米拉病的主要原因，负责家禽业的重大经济损失。 APEC Serogroups O1，O2和O78主要与疾病相关。已经显示脂多糖（LPS）O-抗原是一种有效的抗原，用于诱导特异性保护性免疫应答。因此，我们试图开发一种多价多糖疫苗，以防止大多数APEC感染。我们之前报道了在减毒的沙门氏菌肠道肠杆菌S740中稳定的质粒PSS27的质粒PSS27的表达（10.8kb）提供了对APEC O1攻击的优异保护。在该研究中，构建了含有APEC O 2 O-抗原多糖基因簇（15.5kb）的质粒pS28。通过蛋白质印迹验证了Salmonella疫苗菌株S740中PSS28编码APEC O 2 O-抗原的生物合成。重组沙门氏菌疫苗菌株S740（PSS28）引发了对鸡型中的毒性野生型APEC O2攻击的同源保护。此外，通过相等体积混合所述两种单价疫苗菌株5740（PSS27）和S740（PSS28），开发针对APEC O1和O2的二价疫苗候选物。用二价疫苗免疫鸡的免疫引发血清IgG和粘膜SIGA抗体的产生抵抗APEC O1和O2的LP。此外，由二价疫苗诱导的抗体促进过Opson化，激发补蛋白介导的杀菌活性，并用毒力APEC O1和O 2菌株引发了致命攻击的保护。这些结果表明，由5740（PSS27）和5740（PSS28）组成的二价疫苗是针对APEC O1和O2感染的有前途的疫苗候选者。 （c）2018年elestvier有限公司保留所有权利。

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  • 来源
    《Vaccine》 |2018年第8期|共9页
  • 作者

    Han Yue; Liu Qing; Willias Stephan; Liang Kang; Li Pei; Cheng Anchun; Kong Qingke;

  • 作者单位

    Sichuan Agr Univ Inst Prevent Vet Med Coll Vet Med Chengdu 611130 Sichuan Peoples R China;

    Southwest Univ Coll Anim Sci &

    Technol Chongqing 400715 Peoples R China;

    Univ Florida Dept Infect Dis &

    Pathol Gainesville FL 32608 USA;

    Sichuan Agr Univ Inst Prevent Vet Med Coll Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Coll Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Coll Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Coll Vet Med Chengdu 611130 Sichuan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    APEC O1 and O2; O-antigen polysaccharide; S. Typhimurium; Bivalent vaccine;

    机译：APEC O1和O2;O-抗原多糖;s。 刺血管;二价疫苗;

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