...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Generation of a recombinant Newcastle disease virus expressing two foreign genes for use as a multivalent vaccine and gene therapy vector
【24h】

Generation of a recombinant Newcastle disease virus expressing two foreign genes for use as a multivalent vaccine and gene therapy vector

机译：生成表达两种外源基因的重组新城疫病毒用作多价疫苗和基因治疗载体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene therapy. A majority of these NDV vectors express only a single foreign gene through either an independent transcription unit (ITU) or an internal ribosomal entry site (IRES). In the present study, we combined the ITU and IRES methods to generate a novel NDV LaSota strain-based recombinant virus vectoring the red fluorescence protein (RFP) and the green fluorescence protein (GFP) genes. Biological assessments of the recombinant virus, rLS/IRES-RFP/GFP, showed that it was slightly attenuated in vivo, yet maintained similar growth dynamics and viral yields in vitro when compared to the parental LaSota virus. Expression of both the RFP and GFP was detected from the rLS/IRES-RFP/GFP virus-infected DF-1 cells by fluorescence microscopy. These data suggest that the rLS/IRES-RFP/GFP virus may be used as a multivalent vector for the development of vaccines and gene therapy agents. Published by Elsevier Ltd.
机译：新城疫病毒（NDV）已被用作疫苗和基因治疗的发展中的载体。这些NDV载体中的大部分仅通过独立的转录单元（ITU）或内部核糖体进入部位（IRES）表达单个外源基因。在本研究中，我们将ITU和IRES方法组合生成新的NDV LASOTA菌株的重组病毒载体矢量载体红荧光蛋白（RFP）和绿色荧光蛋白（GFP）基因。重组病毒，RLS / IRES-RFP / GFP的生物评估表明它在体内略微衰减，而与父母的Lasota病毒相比，体外，在体外保持了类似的生长动力学和病毒产量。通过荧光显微镜从RLS / IRES-RFP / GFP病毒感染的DF-1细胞中检测RFP和GFP的表达。这些数据表明RLS / IRES-RFP / GFP病毒可以用作多价向量以进行疫苗和基因治疗剂的开发。 elsevier有限公司出版

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号