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首页> 外文期刊>Vaccine >Development of multi-epitope driven subunit vaccine in secretory and membrane protein of plasmodium falciparum to convey protection against malaria infection
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Development of multi-epitope driven subunit vaccine in secretory and membrane protein of plasmodium falciparum to convey protection against malaria infection

机译：疟原虫分泌和膜蛋白的多表位驱动亚基疫苗的发展传达疟疾感染保护

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摘要

Malaria infection is the severe health concern for a long time. As per the WHO reports, the malarial infection causes huge mortality all around the world and is incomparable with any other infectious diseases. The absence of effective treatment options and increasing drug resistance to the available therapeutics like artemisinin and other derivatives demand an efficient alternative to overcome this death burden. Here, we performed the literature survey and sorted the Plasmodium falciparum secretory and membrane proteins to design multi-epitope subunit vaccine using an adjuvant, B-cell- and T-cell epitopes. Every helper T-lymphocyte (HTL) epitope was IFN-gamma positive and IL-4 non-inducer. The physicochemical properties, allergenicity, and antigenicity of designed vaccine were analyzed for the safety concern. Homology modeling and refinement were performed to obtain the functional tertiary structure of vaccine protein followed by its molecular docking with the toll-like receptor-4 (TLR-4) immune receptor. Molecular dynamics simulation was performed to check the interaction and stability of the receptor-ligand complex. Lastly, in silico cloning was performed to generate the restriction clone of designed vaccine for the futuristic expression in a microbial expression system. This way, we designed the multiepitope subunit vaccine to serve the people living in the global endemic zone. (C) 2018 Elsevier Ltd. All rights reserved.
机译：疟疾感染是严重的健康问题很长一段时间。根据世卫组织报道，疟疾感染导致全世界的巨大死亡率，并对任何其他传染病无可比拟。没有有效的治疗方案和对可用治疗的药物抵抗，如青蒿素和其他衍生物等有效的替代方案，以克服这种死亡负担。在这里，我们进行了文献调查，并将疟原虫分泌物和膜蛋白分类为使用佐剂，B细胞和T细胞表位设计多表位亚基疫苗。每个辅助T淋巴细胞（HTL）表位为IFN-Gamma阳性和IL-4非诱导剂。分析了设计疫苗的物理化学性质，过敏性和抗原性以进行安全问题。进行同源性建模和改进，以获得疫苗蛋白的功能性三相结构，然后用其分子对接与Toll样受体-4（TLR-4）免疫受体进行分子对接。进行分子动力学模拟以检查受体 - 配体络合物的相互作用和稳定性。最后，在进行硅克隆中，进行用于在微生物表达系统中的未来派表达的设计疫苗的限制克隆。通过这种方式，我们设计了多态亚疫苗，以服务于生活在全球流行区的人们。 （c）2018年elestvier有限公司保留所有权利。

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