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首页> 外文期刊>Vaccine >Examination of antigenic proteins of ce:italic>Trypanosoma cruzi/ce:italic> to fabricate an epitope-based subunit vaccine by exploiting epitope mapping mechanism
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Examination of antigenic proteins of ce:italic>Trypanosoma cruzi/ce:italic> to fabricate an epitope-based subunit vaccine by exploiting epitope mapping mechanism

机译：检查＆lt中抗原蛋白的检查：斜斜肌瘤Cruzi＆ / ce：斜体>通过利用表位映射机制来制造基于表位的亚基疫苗

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Chagas disease is a protozoan parasitic disease caused byTrypanosoma cruzi.This injurious disease spread by the circulation of the blood sucking triatomine insects and transmitted to humans. Chagas disease is endemic in Latin America and also known as American trypanosomasis. Currently, 7 million people are infected byT. cruziinfection and about 22,000 death cases were reported per year throughout the Americas. Various immunization approaches againstT. cruziinfection have been examined and some of the developed vaccine showed efficacy in animal models but there is no effective and safe vaccines for humans have been developed yet. Since, the drug resistance is increasing day by day because the developed drug (nifurtimox and benznidazole) to controlT. cruziinfection, failed to activate a prodrug and still no drug and vaccine have been established. To control the infection of chagas disease, here in this study we use immunoinformatics method to design multi-epitope subunit vaccine againstT. cruziinfection. Moreover, on the basis of immunogenicity B and T cell epitopes were evaluated. The allergenicity, antigenicity was predicted to ensure the safety of vaccine constructs whereas, the physiochemical property showing the stable nature of final vaccine model. Further, molecular docking was performed to optimize the interaction between TLR-2 and TLR-4 (receptor) and vaccine model (ligand) complex. Molecular dynamics simulation was performed to evaluate the energy minimization; RMSD and RMSF plot which confirm the stability of TLR-2 and TLR-4 (receptor) present on immune cells and vaccine model (ligand) complex. This study needed the experimental validation for the safety and immunogenic behavior of designed vaccine protein and it may be helpful in future to controlT. cruziinfection.
机译：Chagas疾病是一种原生动物寄生疾病，导致Bytypanosoma Cruzi。这种有害疾病被血液血液吸入三角虫昆虫并传播给人类。 Chagas疾病是拉丁美洲的地方性，也称为美国锥虫。目前，700万人被感染了。整个美洲每年报告克鲁齐林和约22,000人死亡病例。各种免疫方法对不起。已经检查了克鲁齐林，并且一些发育的疫苗在动物模型中表现出疗效，但尚未开发有效且安全的疫苗。由于，由于发育的药物（Nifurtimox和Benznidazole）来控制，耐药性日益增加。 Cruziinfection，未能激活前药，仍然没有制定药物和疫苗。为了控制Chagas病的感染，在这项研究中，我们使用免疫信息方法来设计多表位亚基疫苗反对。 Cruziinfection。此外，基于免疫原性B和T细胞表位的基础。预测过敏性，抗原性，以确保疫苗构建体的安全性，而呈现出最终疫苗模型的稳定性质的生理化学性能。此外，进行分子对接以优化TLR-2和TLR-4（受体）和疫苗模型（配体）复合物之间的相互作用。进行分子动力学模拟以评估能量最小化; RMSD和RMSF图，其证实了免疫细胞和疫苗模型（配体）复合物上存在的TLR-2和TLR-4（受体）的稳定性。本研究需要对设计疫苗蛋白的安全性和免疫原性行为进行实验验证，将来可能有助于控制。 Cruziinfection。

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