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首页> 外文期刊>Vaccine >Oral administration of coated PEDV-loaded microspheres elicited PEDV-specific immunity in weaned piglets
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Oral administration of coated PEDV-loaded microspheres elicited PEDV-specific immunity in weaned piglets

机译：口服涂覆的PEDV加载的微球引发了断奶仔猪的PEDV特异性免疫

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摘要

Porcine epidemic diarrhea virus (PEDV) infects pigs of all ages by invading villous epithelial cells of the small intestine causing severe diarrhea with high mortality rate in suckling piglets. Mucosal immunity is believed to play an important role in PEDV control and mucosal delivery of vaccines induces mucosal immunity more efficiently than parenteral vaccination. In this study, coated PEDV-loaded microspheres with the size range of 700-900 mu m in diameter were developed by centrifugal granulation-fluidized bed coating and demonstrated as an effective oral delivery system to protect PEDV antigens against the complex gastrointestinal environment by detecting the live virus particles in microspheres after the simulated gastric fluid treatment and the PEDV RNA in fecal swabs collected from all weaned piglets (100%) orally inoculated with coated PEDV-loaded microspheres. Weaned piglets orally immunized with coated PEDV-loaded microspheres developed higher levels of PEDV-specific antibodies (IgG and IgA) in their sera and saliva than those negative control groups (p & 0.001 or p & 0.01). Furthermore, neutralization assays demonstrated that serum antibodies in coated PEDV-loaded microspheres groups could significantly inhibit virus infection in Vero cells, compared to PEDV only group (p & 0.05). Overall, our results indicate that the coated PEDV-loaded microspheres might serve as an effective way to induce PEDV-specific mucosal immunity in pigs against PEDV. (C) 2018 Elsevier Ltd. All rights reserved.
机译：猪流行性腹泻病毒（PEDV）通过侵入小肠的绒毛上皮细胞来感染所有年龄的猪，导致严重的腹泻，在哺乳仔猪中具有高死亡率。据信粘膜免疫在PEDV控制中发挥着重要作用，并且疫苗的粘膜递送比肠胃外疫苗更有效地诱导粘膜免疫。在该研究中，通过离心造粒流化床涂层开发了尺寸范围为700-900μmm的涂覆的Pedv-Loaded微球，并作为一种有效的口服递送系统，通过检测到致态胃肠环境来保护PEVV抗原对复杂的胃肠环境。在模拟胃液处理后微球中的活病毒颗粒和从所有断奶仔猪（100％）收集的粪便拭子中的PEDV RNA（100％）用涂覆的PEVV负载的微球接种。用涂覆的PEDV负载的微球口服免疫的断奶仔猪在其血清和唾液中产生更高水平的PEDV特异性抗体（IgG和IgA），而不是那些阴性对照组（P＆amp; 0.001或P＆amp; 0.01）。此外，与PEDV仅组（P＆amp; 0.05）相比，中和测定表明，涂覆的Pedv载荷的微球中的血清抗体可以显着抑制Vero细胞中的病毒感染。总体而言，我们的结果表明涂覆的PEVV负载的微球可能是诱导猪患有PECV的PEDV特异性粘膜免疫的有效方法。 （c）2018年elestvier有限公司保留所有权利。

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  • 来源
    《Vaccine》 |2018年第45期|共7页
  • 作者

    Wen Zhifen; Xu Zhichao; Zhou Qingfeng; Li Wei; Wu Yunyan; Du Yunping; Chen Li; Zhang Yun; Xue Chunyi; Cao Yongchang;

  • 作者单位

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Wens Foodstuffs Grp Co Ltd Wens Grp Acad Xinxing 527400 Guangdong Peoples R China;

    Wens Foodstuffs Grp Co Ltd Wens Grp Acad Xinxing 527400 Guangdong Peoples R China;

    Wens Foodstuffs Grp Co Ltd Wens Grp Acad Xinxing 527400 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

    Sun Yat Sen Univ Sch Life Sci State Key Lab Biocontrol Guangzhou 510006 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    Porcine epidemic diarrhea virus (PEDV); Vaccine; Coated PEDV-loaded microspheres; Weaned piglets;

    机译：猪流行性腹泻病毒（PEDV）;疫苗;涂层PEDV加载的微球;断奶仔猪;

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