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首页> 外文期刊>Vaccine >Saccharospirillum correiae sp nov., an endophytic bacterium isolated from the halophyte Halimione portulacoides
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Saccharospirillum correiae sp nov., an endophytic bacterium isolated from the halophyte Halimione portulacoides

机译：SacCharospirillum Correiae SP 11月。来自烟道嗜酸盐的内皮细菌细菌，嗜睡症

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摘要

A Gram-stain negative, oxidase-and catalase-positive, motile, aerobic, non-pigmented spirillum, designated CPA1(T), was isolated from The surface-sterilized tissues of a halophyte, Halimione portulacoides, collected from a salt marsh in Aveiro, Portugal. The isolate was mesophilic, facultatively alkaliphilic and halophilic, and grew between 18 and 42.5 degrees C (optimum 30 degrees C), from pH 5.0 to 11.5 (optimum 7.0-7.5), from 0.5 to 5% NaCl (w/v, optimum 2 %). Analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Saccharospirillum, as the highest sequence similarities were observed with Saccharospirillum impatiens EL-105(T) (96.46 %), Saccharospirillum salsuginis YIM-Y25(T) (96.32 %) and Saccharospirillum aestuarii IMCC 4453(T) (95.17 %). The next closest matches were with other genera and below 95.0 %. Phylogenetic analyses revealed that the strain forms a robust clade with other species of the genus Saccharospirillum. The main respiratory quinone was Q-8 and the major fatty acids were C-16 : 0 and summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c). The DNA G+ C content was 55.2 mol%. Molecular, physiological and biochemical differences between strain CPA1(T) and other type strains of species of the genus Saccharospirillum support the addition of this novel species to the genus, and the name Saccharospirillum correiae sp. nov. is proposed, with CPA1(T) (= CECT 9131(T) = LMG 29516(T)) as the type strain.
机译：从Aveiro的盐沼收集的表面灭菌组织中分离出分离出来的CPA1（T）的革兰氏染色的阴性，氧化酶和过氧化氢酶阳性，氧化酶和过氧化氢酶阳性，动机，有氧酶，指定的CPA1（t），从盐沼收集， 葡萄牙。分离物是嗜苯胺，散骨碱性和嗜盐，并在pH 5.0至11.5（最佳7.0-7.5），从0.5-5％NaCl（W / V，最佳2，最佳30℃）（最佳30℃）介于18至42.5℃（最佳30℃）之间的增长％）。 16S rRNA基因序列的分析表明，该菌株属于Saccharospirillum属，因为蔗糖棘毛虫El-105（96.46％），SacCharospirillum Salsuginis yim-Y25（96.32％）观察到最高序列相似之处（96.46％）（96.32％）和SacCharospirillum Aestuarii IMCC 4453（T）（95.17％）。下一个最接近的比赛与其他属，低于95.0％。系统发育分析显示，该菌株与其他物种的萨克斯季兆子组成强大的疏水性。主要呼吸醌是Q-8，主要脂肪酸是C-16：0和总结特征8（C-18：1 Omega 7C和/或C-18：1 Omega 6c）。 DNA G + C含量为55.2摩尔％。菌株CPA1（T）和Saccharospirill属的其他类型菌株之间的分子，生理和生物化学差异支持对属的添加这种新种类，以及SacCharospirillum Correiae Sp。 11月。提出了CPA1（T）（= CECT 9131（T）= LMG 29516（T））作为类型应变。

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