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Pseudorhodobacter ponti sp nov., isolated from seawater

机译：Ponti Sp oc1月。，与海水隔离

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摘要

A Gram-stain-negative, short-rod-shaped, non-motile bacterial strain, designated HWR-46(T), was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain HWR-46 T grew optimally at pH 7.0-8.0, at 20-25 degrees C and in the presence of 2-3% (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HWR-46(T) fell within the clade comprising Pseudorhodobacter species, clustering with the type strain of Pseudorhodobacter aquimaris, with which it exhibited 98.4% sequence similarity. 16S rRNA gene sequence similarity between strain HWR-46(T) and the type strains of other Pseudorhodobacter species was 95.1-96.3 %. The DNA G+ C content of strain HWR46(T) was 63.1 mol% and its mean DNA-DNA relatedness value with P. aquimaris HDW-19(T) was 24.6 +/- 1.5 %. Strain HWR-46(T) contained only Q-10 as the ubiquinone and summed feature 8 (C(18 : 1 omega)7c and/or C-18 : 1 omega 6c) as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and an unidentified aminophospholipid. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain HWR-46(T) is distinguishable from other Pseudorhodobacter species. On the basis of the data presented, strain HWR-46(T) is considered to represent a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter ponti sp. nov. is proposed. The type strain is HWR-46(T) (= KCTC 52470(T) = NBRC 112426(T)).
机译：从黄海，韩国海水中分离出革兰氏染色阴性，短杆形的非运动细菌菌株，被分离出来的海水，并进行了多阵列分类学研究。菌株HWR-46 T在pH 7.0-8.0中最佳地增长，在20-25摄氏度下，在2-3％（w / v）NaCl存在下。基于16S rRNA基因序列的邻近的系统发育树表明，菌株HWR-46（T）落入包含伪发病杆菌的患者内，与伪发泡杆菌的类型菌株聚集，其表现出98.4％的序列相似性。 16S菌株HWR-46（T）之间的rRNA基因序列相似性和其他伪发泡杆菌的类型菌株为95.1-96.3％。菌株HWR46（T）的DNA G + C含量为63.1摩尔％，其平均DNA-DNA相关性值与P. Aquimaris HDW-19（T）为24.6 +/- 1.5％。菌株HWR-46（T）仅含有Q-10作为泛醌，并且总结特征8（C（18：1ω）7c和/或C-18：1ω6c）作为主要脂肪酸。主要极性脂质是磷脂酰胆碱，磷脂酰甘油，未识别的氨基吡咯和未识别的氨基磷脂。差分表型性质与系统发育的独特性一起证明了菌株HWR-46（T）可区分伪发酵杆菌。在所呈现的数据的基础上，菌株HWR-46（T）被认为是代表伪发电术会的新颖物种，名称伪杆菌神杆菌名称。 11月。提出。类型应变为HWR-46（t）（= kctc 52470（t）= nbrc 112426（t））。

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