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首页> 外文期刊>Vaccine >Transfer of Pseudomonas pictorum Gray and Thornton 1928 to genus Stenotrophomonas as Stenotrophomonas pictorum comb. nov., and emended description of the genus Stenotrophomonas
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Transfer of Pseudomonas pictorum Gray and Thornton 1928 to genus Stenotrophomonas as Stenotrophomonas pictorum comb. nov., and emended description of the genus Stenotrophomonas

机译:将Pseudomonas Pictorum Grey和Thornton 1928转移到Stenotrophoomonas属的Stenotrophomonas pictorum梳子。 11月。,并强化史胸甲孢子属的化合物描述

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A polyphasic taxonomic approach including analysis of phenotypic, physiological and genotypic characteristics, 16S rRNA gene sequence and DNA-DNA hybridization analysis was used to determine the most consistent affiliation of Pseudomonas pictorum. Pseudomonas pictorum ATCC 23328 T exhibited phenotypic traits of members of the genus Stenotrophomonas including cellular fatty acid composition, quinone and limited range of substrates that could be used. Antibiotic susceptibility and physiological characteristics were determined. The DNA G+C content was 65.7 mol%. Phylogenetic analysis revealed that the type strains of Stenotrophomonas terrae, Stenotrophomonas humi, Stenotrophomonas nitritireducens and Stenotrophomonas acidaminiphila were the nearest relatives (16S rRNA gene sequence similarity of 98.0 to 98.8 %). All the other type strains of species of the genus Stenotrophomonas showed high 16S rRNA gene sequence similarities (96.8 to 97.2 %). DNA-DNA hybridizations revealed 31.0, 32.0, 43.3 and 43.6% reassociation between Pseudomonas pictorum ATCC 23328 T and the type strains of S. terrae, S. humi, S. nitritireducens and S. acidaminiphila, respectively. Our overall results indicate that Pseudomonas pictorum should be transferred to the genus Stenotrophomonas as a novel species of this genus, Stenotrophomonas pictorum comb. nov. Since the original description of the genus Stenotrophomonas was made with only one species (Stenotrophomonas maltophilia), an emendation of the genus description is proposed in order to match better with the characteristics of the eleven novel species assigned to this genus since then.
机译:一种多相分类方法,包括分析表型,生理学和基因型特征,16S rRNA基因序列和DNA-DNA杂交分析,用于确定假单胞菌皮卡抑制的最一致的隶属关系。 Pseudomonas pictorum ATCC 23328 T表现出斯内诺科托纳斯属的成员的表型性状,包括可以使用的细胞脂肪酸组成,醌和有限的基材。确定了抗生素敏感性和生理特性。 DNA G + C含量为65.7mol%。系统发育分析表明,斯内特科莫氏菌,斯科博洛莫氏菌,Stenotophomonas,Stenotophomonas硝基菌和Stenotrophomonas acidaminiphila的类型菌株(16s rRNA基因序列相似度为98.0至98.8%)。所有其他类型的史胸育霉菌属种类株高16s rRNA基因序列相似性(96.8至97.2%)。 DNA-DNA杂交分别揭示了Pseudomonas pictorum ATCC 23328 T和S. Terrae,S. Humi,S.硝基菌和S. acidaminiphila之间的31.0,32.0,43.3和43.6%的重组。我们的整体结果表明,假单胞菌皮卡索姆应转移到斯滕科诺斯属,作为该属的新种类,Stenotrophomonasictum梳子。 11月。由于仅具有一种物种(Stenotrophomonas麦芽粒子)的原始描述,所以提出了属性描述的修正,以便更好地与此后分配给该属的11种新颖物种的特征。

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