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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >The effect of isomerism and other structural variations on the G-quadruplex DNA-binding properties of some nickel Schiff base complexes
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The effect of isomerism and other structural variations on the G-quadruplex DNA-binding properties of some nickel Schiff base complexes

机译：异构性和其他结构变化对一些镍席夫碱基复合物的G-Quadflex DNA结合特性

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A series of novel isomeric nickel Schiff base complexes, as well as nickel complexes of related ligands having asymmetric structures have been prepared and characterised using microanalysis, H-1 and C-13 NMR spectroscopy and ESI-MS. The Schiff base ligands were prepared by condensation reactions involving ethylenediamine and different derivatives of benzophenone. The solid-state structures of eight of the complexes were also determined and revealed that each possessed a regular square planar coordination geometry around the metal ion. Many of the new complexes featured at least one, and in many instances two, protonatable pendant groups that enhance aqueous solubility. This enabled the DNA binding properties of the latter complexes to be explored using a variety of instrumental approaches, including ESI-MS, circular dichroism (CD) spectroscopy, FRET melting assays and FID assays, as well as molecular docking studies. The results of experiments performed using ESI-MS suggested that none of the nickel complexes exhibit a high affinity towards either a double stranded DNA (dsDNA) molecule D2, or the parallel unimolecular quadruplex DNA (qDNA) molecule Q1. In contrast, complexes (8) and (12) both gave spectra which reflected a significant level of binding to the parallel tetramolecular qDNA Q4. The results of binding experiments performed using CD spectroscopy suggested that (12) exhibits a significant level of affinity towards most types of DNA, while (4) shows a preference for interacting with parallel, unimolecular qDNA molecules. Complex (4) produced the lowest values of DC50 in FID assays performed using parallel Q1 or Q4, confirming its affinity for these qDNA molecules. The results of FRET melting experiments provided further evidence that (12), along with (8), can interact extensively with anti-parallel unimolecular qDNA. Experiments which monitored the effect of the nickel complexes on the melting temperature of D2 showed that none had a stabilising effect on this dsDNA molecule.
机译：已经制备了一系列新的异构镍席基孢子基络合物，以及具有不对称结构的相关配体的镍复合物，并使用微扫描，H-1和C-13 NMR光谱和ESI-MS为特征。席夫碱配体通过包括乙二胺和二苯甲酮的衍生物不同的缩合反应制备。还确定了八个复合物的固态结构，并揭示各自在金属离子周围具有规则的平面平面配位几何形状。许多新复合物在许多情况下具有至少一个，并且在许多情况下，其两种蛋白质悬浮液增强含水溶解度。这使得后者复合物的DNA结合特性使用各种仪器方法来探索，包括ESI-MS，圆形二色性（CD）光谱，FRET熔化测定和FID测定以及分子对接研究。使用ESI-MS进行的实验结果表明，镍络合物中没有朝向双链DNA（DSDNA）分子D2或平行的单分子QuadrepleDNA（QDNA）分子Q1的高亲和力。相反，复合物（8）和（12）两者都有光谱，其反映了与平行四分子QDNA Q4的显着含量的结合。使用CD光谱进行的结合实验结果表明（12）对大多数类型的DNA表现出显着的亲和力，而（4）表示与平行相互作用的QDNA分子相互作用的偏好。复合物（4）在使用平行Q1或Q4进行的FIF测定中产生DC50的最低值，确认其对这些QDNA分子的亲和力。 FRET熔化实验的结果提供了进一步的证据：（12）以及（8）可以广泛地与抗平行解压缩QDNA相互作用。监测镍络合物对D2熔化温度的实验表明，没有对该DSDNA分子的稳定作用。

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