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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Facile preparation of multifunctionalisable 'stealth' upconverting nanoparticles for biomedical applications
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Facile preparation of multifunctionalisable 'stealth' upconverting nanoparticles for biomedical applications

机译：用于生物医学应用的多功能性“隐形”纳米粒子的舒适性

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Pure hexagonal (beta-phase) NaYF4-based hydrophobic upconverting nanoparticles (UCNPs) were surfacemodified with O-phospho-L-threonine (OPLT), alendronic acid, and PEG-phosphate ligands to generate water-dispersible UCNPs. Fourier-transform infrared (FTIR) spectroscopy was used to establish the presence of the ligands on the UCNP surface. These UCNPs exhibit great colloidal stability and a near-neutral surface at physiological pH, as confirmed by dynamic light scattering (DLS) and zeta potential (zeta) measurements, respectively. The particles also display excellent long-term stability, with no major adverse effect on the size of UCNPs when kept at pH 7.4. Upon exposure to human serum, PEG-phosphate- and alendronate-coated UCNPs showed no formation of biomolecular corona, as confirmed by SDS-PAGE analysis. The photophysical properties of water-dispersible UCNPs were investigated using steady-state as well as time-resolved luminescence spectroscopy, under excitation at ca. 800 nm. The results clearly show that the UCNPs demonstrate bright upconversion (UC) luminescence. Furthermore, the presence of reactive groups on the NPs, such as free amine groups in alendronate-coated UCNPs, enables further functionalisation of UCNPs with, for example, small molecules, peptides, proteins, and antibodies. Overall these protein corona resistant UCNPs show great biocompatibility and are worthy of further investigation as potential new biomaging probes.
机译：纯六边形（β相）基于NayF 4的疏水性上变化纳米颗粒（UCNP）用O-磷酸-1-苏氨酸（OPLT），醇酸和PEG磷酸盐配体进行了表面上滴血，以产生水分散性UCNP。傅里叶变换红外（FTIR）光谱法用于建立UCNP表面上的配体的存在。这些UCNP分别在生理pH下表现出较大的胶体稳定性和近中性表面，通过动态光散射（DLS）和Zeta电位（Zeta）测量来证实。颗粒还显示出优异的长期稳定性，在保持在pH 7.4时，对UCNP的大小没有主要不利影响。暴露于人血清后，PEG-磷酸酯和丙烯酸酯涂覆的UCNPS没有形成生物分子电晕，如SDS-PAGE分析所证实。在CA的激发下，使用稳态以及时间分辨的发光光谱研究了水分散性UCNP的光物理性质。 800 nm。结果清楚地表明，UCNP展示了明亮的上变化（UC）发光。此外，在NPS上存在反应性基团，例如丙烯酸酯涂覆的UCNP中的游离胺基，可以进一步官能化UCNP与例如小分子，肽，蛋白质和抗体。总体而言，这些蛋白质电晕抗性UCNPS表现出很大的生物相容性，值得进一步调查作为潜在的新生物探头。

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    Nsubuga Anne; Sgarzi Massimo; Zarschler Kristof; Kubeil Manja; Huebner Rene; Steudtner Robin; Graham Bim; Joshi Tanmaya; Stephan Holger;

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    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Ion Beam Phys &

    Mat Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Resource Ecol Bautzner Landstr 400 D-01328 Dresden Germany;

    Monash Univ Monash Inst Pharmaceut Sci Parkville Vic 3052 Australia;

    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

    Helmholtz Zentrum Dresden Rossendorf Inst Radiopharmaceut Canc Res Bautzner Landstr 400 D-01328 Dresden Germany;

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  • 中图分类 化学;无机化学;
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