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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Binding of Al(III) to synthetic RNA and metal-mediated strand aggregation
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Binding of Al(III) to synthetic RNA and metal-mediated strand aggregation

机译：Al（III）与合成RNA和金属介导的链聚集的结合

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Over the last few years, focused interest in aluminum has been heightened by recent studies regarding its health effects. Its possible relation with chronic diseases makes it convenient to address more in depth the reactivity of aluminum with biologically relevant molecules. The present work investigates the interaction of the aluminum ion with two synthetic RNAs, poly(rA) and poly(rU), through a detailed thermodynamic and kinetic study. The trivalent aluminum ion was kept in solution by complexation with the cacodylate anion, even at neutral pH, thus making the study with biological molecules feasible. The results obtained by spectrophotometry, circular dichroism, viscometry and thermal stability measurements indicate that aluminium strongly interacts with single and duplex RNA structures. The kinetic experiments point out that, even though cacodylate is required to keep the metal in solution, it actually inhibits the reaction of aluminum with RNA as it converts the metal into an unreactive dimer species. Notably, further interaction occurred in an excess of the aluminum/cacodylate complex, inducing aggregation of single-stranded RNAs. An analysis of the kinetic data has shown that the modes of aggregation of the two RNAs differ and such a difference can be ascribed to the diverse polynucleotide secondary structures. The observed stabilization of multiple-stranded systems by aluminum can serve as a model for future studies due to the interest aroused by this metal in the study of non-canonical nucleic acid structures.
机译：在过去的几年里，最近关于其健康影响的研究已经提高了铝的兴趣。它与慢性疾病的可能关系使得可以方便地通过对生物相关分子进行更深的铝的反应性。本作通过详细的热力学和动力学研究，研究了铝离子与两个合成RNA，聚（Ra）和聚（Ru）的相互作用。即使在中性pH下，通过与Cacocylylate阴离子络合而保持三价铝离子，从而使得具有生物分子的研究。通过分光光度法，圆形二色性，粘度测定和热稳定性测量获得的结果表明铝强烈地与单层RNA结构相互作用。动力学实验指出，即使使性差异化在溶液中保持金属，它实际上抑制了铝与RNA的反应，因为它将金属转化为非反应性二聚体物质。值得注意的是，进一步的相互作用在过量的铝/卵卵络合物复合物中发生，诱导单链RNA的聚集。对动力学数据的分析表明，两个RNA的聚集方式不同，并且这种差异可以归因于不同的多核苷酸二次结构。观察到通过铝的多链系统的稳定可以作为未来研究的模型，因为这种金属在非规范核酸结构的研究中引起的兴趣。

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