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首页> 外文期刊>Current Protocols in Cell Biology >UNIT 10.9 Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts
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UNIT 10.9 Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts

机译：UNIT 10.9培养培养的和原代成纤维细胞产生的细胞外基质

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摘要

Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from fibroblastic cells; the matrix is three-dimensional, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically.
机译：成纤维细胞分泌并组织细胞外基质（ECM），除了调节诸如生长和存活等细胞功能外，还为它们的粘附，迁移和组织组织提供结构支持。细胞与基质的相互作用对于脊椎动物的发育至关重要。这些过程中的疾病与纤维化，发育畸形，癌症和其他疾病有关。本单元描述了一种制备源自成纤维细胞的三维矩阵的方法。基质是三维的，无细胞和碎片，并附着在二维培养表面上。在这些基质上细胞粘附和扩散是正常的。该基质也可以被压缩成二维基质并被溶解以进行生化研究。

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