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UNIT 10.20 Generation of 3D Collagen Gels with Controlled Diverse Architectures

机译：具有受控多种结构的UNIT 10.20代3D胶原蛋白凝胶

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摘要

Rat tail collagen solutions have been used as polymerizable in vitro three dimensional (3D) extracellular matrix (ECM) gels for single and collective cell migration assays as well as spheroid formation. Factors such as ECM concentration, pH, ionic concentration, and temperature can alter collagen polymerization and ECM architecture. This unit describes how to generate 3D collagen gels that have distinct architectures ranging from a highly reticular meshwork of short thin fibrils with small pores to aloose matrix consisting of stiff, parallel-bundled long fibrils by changing collagen polymerization temperature. This permits analysis of 3D cell migration in different ECM architectures found in vivo while maintaining a similar ECM concentration. Alsoincluded are collagen labeling techniques helpful for ECM visualization during live fluorescence imaging.
机译：大鼠尾部胶原蛋白溶液已用作可聚合的体外三维（3D）细胞外基质（ECM）凝胶，用于单个和集体细胞迁移测定以及球体形成。 ECM浓度，pH，离子浓度和温度等因素会改变胶原蛋白的聚合和ECM结构。本单元介绍如何通过改变胶原蛋白聚合温度来生成具有独特结构的3D胶原蛋白凝胶，其结构范围从具有小孔的短而细的原纤维的高网状网状结构到由坚硬，平行捆扎的长原纤维组成的疏松基质。这允许在体内发现的不同ECM架构中分析3D细胞迁移，同时保持类似的ECM浓度。还包括有助于实时荧光成像期间ECM可视化的胶原蛋白标记技术。

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