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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Anti-Inflammatory and Antioxidant Activities from the Basolateral Fraction of Caco-2 Cells Exposed to a Rosmarinic Acid Enriched Extract
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Anti-Inflammatory and Antioxidant Activities from the Basolateral Fraction of Caco-2 Cells Exposed to a Rosmarinic Acid Enriched Extract

机译：来自暴露于罗马替酸富集提取物的CaCo-2细胞的基底外侧部分的抗炎和抗氧化活性

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摘要

The potential use of Origanum majorana L. as a source of bioavailable phenolic compounds, specifically rosmarinic acid (RA), has been evaluated. Phenolic bioavailability was tested using an in vitro digestion process followed by a Caco-2 cellular model of intestinal absorption. The high-performance liquid chromatography-photodiode array detector-tandem mass spectrometry (HPLC-PAD-MS/MS) analysis showed the main components in the extract were 6-hydroxyluteolin-7-O-glucoside and rosmarinic acid, followed by luteolin-O-glucoside. After digestion process, the amount of total phenolic compounds (TPC) only decreased slightly, although a remarkable reduction in RA (near 50%) was detected. Bioavailable fraction contained 7.37 +/- 1.39 mg/L digested extract of RA with small quantities of lithospermic acid and diosmin and presented an important antioxidant activity (0.89 +/- 0.09 mmol Trolox/L digested extract). Besides, this bioavailable fraction produced a significant inhibition in TNF-alpha, IL-1 beta, and IL-6 secretion, using a human THP-1 macrophages model. Therefore, RA content in the basolateral compartment could play an important role in the antioxidant and anti-inflammatory activities found.
机译：已经评估了ovinganum Majorana L.作为生物可利用酚类化合物的来源，特别是甘氨酸（RA）。使用体外消化过程测试酚醛化生物利用度，然后进行肠道吸收的Caco-2细胞模型。高效液相色谱 - 光电二极管阵列检测器 - 串联质谱（HPLC-PAD-MS / MS）分析显示提取物中的主要成分是6-羟基uTEMOLIN-7-O-葡糖苷和玫瑰花酸，其次是叶黄素-O -Glucoside。消化过程后，总酚类化合物（TPC）的量仅略微降低，尽管检测到Ra（接近50％）的显着降低。生物可利用率含有7.37 +/- 1.39 mg / L消化的Ra消化提取物，少量岩孢菌酸和二孢菌素，并呈现了重要的抗氧化活性（0.89 +/- 0.09mmol Trolex / L消化提取物）。此外，这种生物可利用率馏分使用人THP-1巨噬细胞模型在TNF-α，IL-1β和IL-6分泌中产生显着抑制。因此，基石室中的RA含量可以在抗氧化剂和发现的抗炎活动中起重要作用。

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