One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis

Zhu Zhu-Jun; Chen Hai-Min; Chen Juan-Juan; Yang Rui; Yan Xiao-Jun

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One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis

机译：通过从吡爹基海兰尼斯克隆的多官能脂氧合酶成C8-C9挥发性香气化合物的一步生物转化物

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摘要

The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg.L-1 yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids. C20:5 was the most suitable substrate among the tested fatty acids. The C8-C9 VOCs were generated in good yields from fatty acids, e.g., 2E-nonenal from C20:4, and 2E,6Z-nonadienal from C20:5. Hydrolyzed oils were also tested as substrates. The reactions mainly generated 2E,4E-pentadienal, 2E-octenal, and 2E,4E-octadienal from hydrolyzed sunflower seed oil, corn oil, and fish oil, respectively. PhLOX showed good stability after storage at 4 degrees C for 2 weeks and broad tolerance to pH and temperature. These desirable properties of PhLOX make it a promising novel biocatalyst for the industrial production of volatile aroma compounds.

机译：从Pyropia海兰尼斯克隆的多官能脂氧基酶磷酸酚醛在大肠杆菌中表达，24.4mg.L-1产率。 Pllox可以催化C18-C22脂肪酸的一步生物转化酸纳在C8-C9挥发性有机化合物（VOC）中，表现出比十八烷烯酸的eicosenophoid和多糖酸的催化效率更高。 C20：5是测试脂肪酸中最合适的底物。将C8-C9 VOC从脂肪酸的良好产率产生，例如，从C20：4和2E，6Z-NoneDienal的2E-NonenAl产生，来自C O 2 M20：5。水解油也被测试为底物。反应主要产生2E，4E-五戊类，2E-octenal和2E，4E-Ocdinal分别由水解的向日葵种子油，玉米油和鱼油。在4℃下储存2周后储存良好的稳定性，并且对pH和温度宽的耐受性。这些理想的Phlox性质使其成为挥发性香气化合物的工业生产的有前途的新型生物催化剂。

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来源
《Journal of Agricultural and Food Chemistry》 |2018年第5期|共9页
作者
Zhu Zhu-Jun; Chen Hai-Min; Chen Juan-Juan; Yang Rui; Yan Xiao-Jun;
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作者单位

Ningbo Univ Key Lab Marine Biotechnol Zhejiang Prov Ningbo 315211 Zhejiang Peoples R China;

Ningbo Univ Key Lab Marine Biotechnol Zhejiang Prov Ningbo 315211 Zhejiang Peoples R China;

Ningbo Univ Key Lab Marine Biotechnol Zhejiang Prov Ningbo 315211 Zhejiang Peoples R China;

Ningbo Univ Key Lab Marine Biotechnol Zhejiang Prov Ningbo 315211 Zhejiang Peoples R China;

Ningbo Univ Key Lab Marine Biotechnol Zhejiang Prov Ningbo 315211 Zhejiang Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类营养卫生、食品卫生;农业科学;
关键词
Pyropia haitanensis; lipoxygenase; hydroperoxide lyase; volatile organic compounds; aroma;

机译：Pyropia haitanensis;脂氧化酶;氢过氧化物裂解酶;挥发性有机化合物;香气;

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专利

1. One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis [J] . Zhu Zhu-Jun, Chen Hai-Min, Chen Juan-Juan, Journal of Agricultural and Food Chemistry . 2018,第5期

机译：通过从吡爹基海兰尼斯克隆的多官能脂氧合酶成C8-C9挥发性香气化合物的一步生物转化物
2. Full-length transcriptome sequences obtained by a combination of sequencing platforms applied to heat shock proteins and polyunsaturated fatty acids biosynthesis in Pyropia haitanensis [J] . Wang Wenlei, Chang Jing, Zheng Hongyan, Journal of Applied Phycology . 2019,第2期

机译：通过施用于热冲击蛋白和多不饱和脂肪酸生物合成的测序平台组合获得的全长转录组序列在Pyropia海兰尼斯
3. Model Studies on the Key Aroma Compounds Formed by an Oxidative Degradation of ω-3 Fatty Acids Initiated by either Copper(ll) Ions or Lipoxygenase [J] . Michaela Hammer, Peter Schieberle Journal of Agricultural and Food Chemistry . 2013,第46期

机译：铜（II）离子或脂氧合酶引发的ω-3脂肪酸氧化降解形成关键香气化合物的模型研究
4. Soybean Oil Biofuel Cell: Utilizing Lipoxygenase Immobilized by Modified NafionR to Catalyze the Oxidation of Fatty Acids for Biofuel Cells [C] . Jeanne L. Kerr, Shelley D. Minteer American Chemical Society . 2008

机译：大豆油生物燃料电池：利用通过改性的Nafionr固定的脂氧合酶来催化生物燃料细胞的脂肪酸的氧化
5. Gemlik Tipi sofral?k Siyah Zeytinlerde u?ucu Aroma Ve sülfür bile?enlerinin Belirlenmesi =Determination of the volatile aroma and sulfur compounds in Gemlik type black table olives [D] . Manav, Hesna Merve. 2021

机译：Gemlik型Sofral？K即使在黑橄榄中也是如此
6. A Lipoxygenase from Red Alga Pyropia haitanensis a Unique Enzyme Catalyzing the Free Radical Reactions of Polyunsaturated Fatty Acids with Triple Ethylenic Bonds [O] . Zhujun Zhu, Feijian Qian, Rui Yang, -1

机译：海藻红藻中的脂氧合酶一种独特的酶具有三烯键的多不饱和脂肪酸的自由基反应
7. A lipoxygenase from red alga Pyropia haitanensis, a unique enzyme catalyzing the free radical reactions of polyunsaturated fatty acids with triple ethylenic bonds. [O] . Zhujun Zhu, Feijian Qian, Rui Yang, 2015

机译：来自红藻pyropia haitanensis的脂氧合酶，一种催化多不饱和脂肪酸与三乙烯键的自由基反应的独特酶。
8. Preliminary Results of a Local Lymph Node Assay and a 28-Day Repeated-Dose Toxicity Study in Rats with Fatty Acids, C-18-Unsaturated, Dimers, Polymers with Tall-oil Fatty Acids, Tetraethylenepentamine and Triethylenetetramine, Compounds with C18-Unsaturated, Fatty Acid Trimers. [R] . 2009

机译：具有脂肪酸，C-18-不饱和，二聚体，具有妥尔油脂肪酸，四乙烯五胺和三乙烯四胺的聚合物，具有C18-不饱和的化合物的局部淋巴结测定和28天重复剂量毒性研究的初步结果，脂肪酸三聚体。

One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis

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