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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Programming a Biofilm-Mediated Multienzyme-Assembly-Cascade System for the Biocatalytic Production of Glucosamine from Chitin
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Programming a Biofilm-Mediated Multienzyme-Assembly-Cascade System for the Biocatalytic Production of Glucosamine from Chitin

机译：编程生物膜介导的次耳组装级联系统，用于从甲壳素生物催化生成葡糖苷的生产

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Chitin is used as an essential raw material for the production of glucosamine (G1cN). In this study, we adopted three key enzymes, isolated from Thermococcus kodakaraensis KOD1, that catalyze the sequential conversion of a-chitin into GlcN and developed a multienzyme-assembly-cascade (MAC) system immobilized in a bacterial biofilm, which enabled a multistep one-pot reaction. Specifically, the SpyTag-SpyCatcher and SnoopTag-SnoopCatcher pairs provided covalent and specific binding force to fix enzymes to the biofilm one by one and assemble close enzyme cascades. The MAC system showed great catalytic activity, converting 79.02 +/- 3.61% of alpha-chitin into GlcN with little byproducts, which was 2.09 times that of GlcN catalyzed by a mixture of pure enzymes. The system also exhibited good temperature and pH stability. Notably, 90% of enzyme activity was retained after 6 rounds of reuse, and appreciable activity remained after 17 rounds.
机译：用甲壳素用作生产葡糖胺（G1CN）的基本原料。 在这项研究中，我们采用了三种关键酶，该酶分离出来自热电偶柯卡拉森斯kod1，该酶催化A-甲壳素的顺序转化为GLCN，并在细菌生物膜中培养了固定在细菌生物膜中的多酶组装 - 级联（MAC）系统，这使得一个MultiSp一体化 -Pot反应。 具体而言，SpyTag-Spycatcher和SnoOptag-Snoopcatcher对提供了共价和特异性结合力，将酶逐一固定到生物膜中并组装近酶级联。 MAC系统显示出具有很大的催化活性，将79.02 +/- 3.61％的α-甲壳素转化为GLCN，副产物很少，其通过纯酶的混合物催化的GLCN的2.09倍。 该系统还表现出良好的温度和pH稳定性。 值得注意的是，90％的酶活性在6轮再利用后保留，并且在17轮后仍然存在明显的活性。

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