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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Food Forensics: Using Mass Spectrometry To Detect Foodborne Protein Contaminants, as Exemplified by Shiga Toxin Variants and Prion Strains
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Food Forensics: Using Mass Spectrometry To Detect Foodborne Protein Contaminants, as Exemplified by Shiga Toxin Variants and Prion Strains

机译：食品取证：使用质谱法检测食源性蛋白质污染物，如滋阴毒素变体和朊病毒菌株所示

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摘要

Food forensicists need a variety of tools to detect the many possible food contaminants. As a result of its analytical flexibility, mass spectrometry is one of those tools. Use of the multiple reaction monitoring (MRM) method expands its use to quantitation as well as detection of infectious proteins (prions) and protein toxins, such as Shiga toxins. The sample processing steps inactivate prions and Shiga toxins; the proteins are digested with proteases to yield peptides suitable for MRM-based analysis. Prions are detected by their distinct physicochemical properties and differential covalent modification. Shiga toxin analysis is based on detecting peptides derived from the five identical binding B subunits comprising the toxin. N-15-labeled internal standards are prepared from cloned proteins. These examples illustrate the power of MRM, in that the same instrument can be used to safely detect and quantitate protein toxins, prions, and small molecules that might contaminate our food.
机译：食品法医师需要各种工具来检测许多可能的食物污染物。 由于其分析灵活性，质谱是其中一种工具。 使用多反应监测（MRM）方法扩大其用于定量以及检测传染性蛋白质（朊病毒）和蛋白质毒素，如滋阴毒素。 样品处理步骤灭活朊病毒和滋生毒素; 用蛋白酶消化蛋白质以产生适于基于MRM的分析的肽。 通过其不同的物理化学性质和差异共价修饰来检测朊病毒。 Shiga毒素分析基于检测衍生自包含毒素的五个相同结合B亚基的肽。 由克隆蛋白质制备N-15标记的内标。 这些实施例说明了MRM的功率，因为相同的仪器可用于安全地检测和定量可能污染我们食物的蛋白质毒素，朊病毒和小分子。

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