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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Positive Regulation of the Transcription of AchnKCS by a bZIP Transcription Factor in Response to ABA-Stimulated Suberization of Kiwifruit
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Positive Regulation of the Transcription of AchnKCS by a bZIP Transcription Factor in Response to ABA-Stimulated Suberization of Kiwifruit

机译:Bzip转录因子响应于ABA刺激的猕猴桃纤维化的ACHNKCs转录的正调节

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摘要

Wound-induced suberization is an essentially protective healing process for wounded fruit to reduce water loss and microbial infection. It has been demonstrated that abscisic acid (ABA) could promote wound suberization, but the molecular mechanism of ABA regulation remains little known. In this study, the transcript level of Achn030011 (designated as AchnKCS), coding a beta-ketoacyl-coenzyme A synthase (KCS) involved in suberin biosynthesis, was found to be significantly upregulated by ABA in wounded kiwifruit. A bZIP transcription factor (Achn270881), a possible downstream transcription factor in the ABA signaling pathway, was screened and designated as AchnbZIP12 according to its homology with related Arabidopsis transcription factors. A yeast one-hybrid assay demonstrated that AchnbZIP12 could interact with the AchnKCS promoter. Furthermore, significant trans-activation of AchnbZIP12 on AchnKCS was verified. The transcript level of AchnbZIP12 was also upregulated upon treatment with ABA. These results imply that AchnbZIP12 acts as a positive regulator in ABA-mediated AchnKCS transcription during wound suberization of kiwifruit.
机译:伤口诱导的芬化是伤口果实的基本保护性愈合过程,以降低水分损失和微生物感染。已经证明,脱离酸(ABA)可以促进伤口均余化,但ABA调节的分子机制仍然众所周知。在该研究中,发现β-酮酰基 - 辅酶的ACHN030011(指定为ACHNKC)的转录物水平,涉及Suberin生物合成的β-酮酰基 - 辅酶A合成酶(KCS)被ABA在受伤的Kiwifruit中显着上调。根据其与相关拟南芥转录因子的同源性,筛选和指定为ABNBZIP12的BZIP转录因子(ACH270881),筛选和指定为Achnbzip12。酵母单杂交测定证明Achnbzip12可以与AchNKC启动子相互作用。此外,验证了AchnKCS上的AchnBzip12的显着越野激活。在用ABA处理时也上调AchnBzip12的转录物水平。这些结果意味着Achnbzip12在ABA介导的ACHNKCS转录中用作KIWIFRUIT的伤口余化的阳性调节剂。

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  • 作者单位

    Shaoxing Univ Sch Life Sci Shaoxing 312000 Zhejiang Peoples R China;

    Zhejiang Univ Key Lab Agroprod Postharvest Handling Zhejiang Key Lab AgroFood Proc Coll Biosyst Engn &

    Food Sci Minist Agr &

    Rural A Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Acad Agr Sci Inst Food Sci Hangzhou 310021 Zhejiang Peoples R China;

    Zhejiang Univ Key Lab Agroprod Postharvest Handling Zhejiang Key Lab AgroFood Proc Coll Biosyst Engn &

    Food Sci Minist Agr &

    Rural A Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Key Lab Agroprod Postharvest Handling Zhejiang Key Lab AgroFood Proc Coll Biosyst Engn &

    Food Sci Minist Agr &

    Rural A Hangzhou 310058 Zhejiang Peoples R China;

    Zhejiang Univ Key Lab Agroprod Postharvest Handling Zhejiang Key Lab AgroFood Proc Coll Biosyst Engn &

    Food Sci Minist Agr &

    Rural A Hangzhou 310058 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    beta-ketoacyl-coenzyme A synthases (KCSs); bZIP; abscisic acid (ABA); kiwifruit; suberization;

    机译:β-酮酰基 - 辅酶是合成酶(KCSS);BZIP;脱硅酸(ABA);Kiwifruit;使用;

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