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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Effect and Mechanism of Elaeagnus angustifolia Flower and Its Major Flavonoid Tiliroside on Inhibiting Non-enzymatic Glycosylation
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Effect and Mechanism of Elaeagnus angustifolia Flower and Its Major Flavonoid Tiliroside on Inhibiting Non-enzymatic Glycosylation

机译：Elaeagnus Angustifolia花及其主要类黄酮类吡罗醇醚对抑制非酶促糖基化的影响及其机制

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摘要

This study aimed to evaluate the antiglycation ability of Elaeagnus angustifolia flower extract and to elucidate the mechanism with its major compound. The results indicated that E. angustifolia flower extract and its major compound tiliroside (24.2 mg/g of extract) exhibited excellent antiglycation ability with inhibition rates of 92.1 and 78.9% at 37.5 mu g/mL, which are much higher than that of aminoguanidine (55.3% at 37.5 mu g/mL). The stable tiliroside-ovalbumin (OVA) complexes were formed through a spontaneous exothermic progress in an equimolar manner, and hydrophobic interaction, hydrogen bond, and van der Waals forces were the major driving forces. Tiliroside could significantly ameliorate the conformation changes of OVA induced by the glycation reaction, quench its fluorescence by a static mechanism, and change the microenvironment adjacent to tryptophan and tyrosine. Molecular docking revealed that tiliroside inserted into the OVA hydrophobic pocket resulted in the formation of five hydrogen bonds. Orbitrap tandem mass spectrometry showed that tiliroside significantly suppressed the glycation of OVA, and the number of glycation sites was reduced from 9 to 5 after tiliroside was added. The above results indicated that E. angustifolia flowers and tiliroside have a good antiglycation effect and can be used as food additives to suppress the undesired glycation reaction during food processing.
机译：本研究旨在评估eLaeagnus Angustifolia花提取物的抗粘性能力，并用其主要化合物阐明机制。结果表明，E.Angustifolia花提取物及其主要化合物硫脲（24.2mg / g提取物）表现出优异的抗粘性能力，抑制率为92.1和78.9％，37.5μg/ ml，远高于氨基胍（ 37.5μmg/ ml的55.3％。通过以等摩尔方式的自发放热进展形成稳定的噻iliLoside-卵磷蛋白（OVA）配合物，疏水相互作用，氢键和范德瓦尔斯力是主要的驱动力。蒂卢罗斯醚可以显着改善糖糖反应诱导的卵子的构象变化，通过静态机制淬灭其荧光，并改变与色氨酸和酪氨酸相邻的微环境。分子对接显示插入OVA疏水袋中的碘化物导致形成五个氢键。 Orbitrap串联质谱表明，蒂卢罗醚显着抑制OVA的糖化，加入碘化硅烷后从9至5降低糖化位点的数量。上述结果表明，E.Angustifolia花和噻oliroside具有良好的抗粘性效果，可用作食品添加剂以抑制食品加工过程中不需要的糖化反应。

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