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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Discovery and Study of Novel Antihypertensive Peptides Derived from Cassia obtusifolia Seeds
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Discovery and Study of Novel Antihypertensive Peptides Derived from Cassia obtusifolia Seeds

机译：Cassia obsusifolia种子的新型抗高血压肽的发现与研究

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摘要

Antihypertensive peptides were screened from thermolysin hydrolysate of Cassia obtusifolia seeds (Jue Ming Zi) using two independent bioassay-guided fractionations, reversed-phase high-performance liquid chromatography (RP-HPLC), and strong cation-exchange (SCX) liquid chromatography coupled with angiotensin I-converting enzyme (ACE) inhibitory assay. The identical peptide in the most active RP-HPLC and SCX fractions was simultaneously de novo sequenced as FHAPWK with high-resolution mass spectrometry. FHAPWK (IC50 = 16.83 +/- 0.90 mu M) was further identified as a competitive inhibitor and a true inhibitor on ACE by a Lineweaver-Burk plot and preincubation experiment, respectively. The molecular docking simulation indicated that FHAPWK could interact with several key residues of the ACE active site, which is consistent with the result of the inhibitory kinetics study. Moreover, its antihypertensive effect was demonstrated using the animal model of spontaneously hypertensive rats. It is concluded that FHAPWK is the first reported antihypertensive peptide derived from thermolysin hydrolysate of C. obtusifolia seeds.
机译：使用两种独立的生物测定的分馏，反相高效液相色谱（RP-HPLC）和强阳离子交换（SCX）液相色谱，筛选抗高血糖蛋白水解产物（Jue Ming Zi）的散热蛋白水解产物筛选血管紧张素I-转换酶（ACE）抑制测定。最活跃的RP-HPLC和SCX级分中的相同肽同时以高分辨率质谱法为FHAPWK进行脱诺。 FHAPWK（IC50 = 16.83 +/-0.90μm）分别通过线织机 - Burk图和预孵育实验进一步鉴定为竞争抑制剂和真正的抑制剂。分子对接模拟表明，FHAPWK可以与ACE活性位点的几个关键残留物相互作用，这与抑制动力学研究的结果一致。此外，使用自发性高血压大鼠的动物模型证明了其抗高血压效果。得出结论，FHAPWK是第一个报告的抗高血压肽衍生自C.渗透溶液种子的热蛋白酶水解产物。

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