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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Thiamethoxam Metabolism and Metabolic Effects in Cell Suspension Culture of Tea (Camellia sinensis L.)
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Thiamethoxam Metabolism and Metabolic Effects in Cell Suspension Culture of Tea (Camellia sinensis L.)

机译：茶氧脲的代谢和茶叶细胞悬浮培养中的代谢效应（Camellia Sinensis L.）

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摘要

Thiamethoxam (TMX) has already been proven to have a physiological effect in plant tissue or cell expect for the insecticidal activity. In our previous study, TMX was verified to be metabolized by tea cells in either a suspension culture or tea plant into several metabolites. Here, tea cell suspension cultures were treated for 45 days to investigate the metabolite effects in both the tea cells and the culture supernatants by nontargeted metabolomics. Using multivariate analysis (PCA and OPLS-DA), all treatment and control groups could be clearly separated. Inside the cells, 113 metabolites were found to be up regulated while 122 were down-regulated, when compared with untreated cells. In the culture supernatant, there were 128 up-regulated and 35 down-regulated metabolites, compared to untreated cultures. KEGG searches revealed that the alanine, aspartate, and glutamate metabolic pathways were strongly affected by TMX metabolism within the tea cell. Molecular docking models showed that (i) 4-aminobutyrate aminotransferase may be related to the formation of 2-chloro-thiazole-5-carboxylic acid and (ii) 3'(2'),5'-bisphosphate nucleotidase may be able to interact with TMX. This study can help us to understand the interaction mechanism of pesticides with plant cells.
机译：噻虫嗪（TMX）已被证明具有植物组织或细胞在期待的杀虫活性生理效应。在我们以前的研究，TMX被证实在是悬浮培养或茶树茶细胞代谢成几种代谢物。在这里，茶叶细胞悬浮培养进行治疗45天，调查在茶叶细胞和非靶向代谢组学培养上清既代谢的影响。使用多变量分析（PCA和OPLS-DA），所有治疗组和对照组，可以清楚地分开。在细胞内部，发现113种代谢物是上调而122是下调，当与未处理的细胞进行比较。在培养上清液中，有128上调和35下调的代谢产物，与未处理的培养物。 KEGG搜索，发现丙氨酸，天门冬氨酸和谷氨酸代谢途径的强烈影响茶叶细胞内代谢TMX。分子对接模型显示，（ⅰ）4-氨基丁酸酯氨基转移酶可能与2-氯 - 噻唑-5-羧酸和（ii）3“（2”）的形成，5'-二磷酸核苷酸酶可能能够相互作用与TMX。这项研究可以帮助我们理解与植物细胞农药的作用机理。

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  • 作者

    Ge Guoqin; Jiao Weiting; Cui Chuanjian; Liao Guangqin; Sun Jun; Hou Ruyan;

  • 作者单位

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

    Anhui Agr Univ Sch Tea &

    Food Sci &

    Technol State Key Lab Tea Plant Biol &

    Utilizat Hefei 230036 Anhui Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    Tea cell suspension culture; Metabolomics; Enzymes; Thiamethoxam;

    机译：茶细胞悬浮培养;代谢组学;酶;祖昔苏姆;

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