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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos
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Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos

机译：用于比色和表面增强拉曼散射感应的敏感和简单的竞争性型纳米甲型测定的三氮杂散射感应

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摘要

The biomimetic enzyme-linked immunosorbent assay (BELISA) is widely used for detection of small-molecule compounds as a result of low cost and reagent stability of molecularly imprinted polymers (MIPs). However, enzyme labels used in BELISA still suffer some drawbacks, such as high production cost and limited stability. To overcome the drawbacks, a biomimetic nanozyme-linked immunosorbent assay (BNLISA) based on MIPs and nanozyme labels was first proposed. For nanozyme labels, platinum nanoparticles (PtNPs) acted as peroxidase by catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into an ideal surface-enhanced Raman scattering (SERS) marker. Blue TMB2+ and bovine serum albumin (BSA)-hapten showed superior selectivity when competing with targets for binding sites on MIPs, named the Pt@ BSA-hapten probe. The BNLISA method was employed to detect triazophos with a limit of detection of 1 ng mL(-1) via colorimetric and SERS methods. Replacing traditional enzymes with nanozymes for combination with MIPs may bring about a new prospect for other compound analyses.
机译：仿生酶联免疫吸附试验（Belisa）广泛用于检测小分子化合物，其由于分子印迹聚合物的低成本和试剂稳定性（MIPS）。然而，Belisa中使用的酶标记仍然遭受一些缺点，例如高生产成本和有限的稳定性。为了克服缺点，首先提出了一种基于MIPS和纳米酶标记的仿生纳米型连接的免疫吸附测定（Bnlisa）。对于纳佐标记，通过将无色3,3'，5,5'-四甲基苯甲酸（TMB）催化成理想的表面增强拉曼散射（SERS）标记，铂纳米颗粒（PTNP）作为过氧化物酶作用为过氧化物酶。蓝色TMB2 +和牛血清白蛋白（BSA）-HAPTEN在竞争MIPS上的绑定网站的目标时显示出优越的选择性，名为PT @ BSA-HAPTEN探头。使用通过比色和SERS方法检测具有1ng ml（-1）的限制的三唑酯。用NaNozymes取代与MIPS的组合的传统酶可能会带来其他化合物分析的新前景。

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  • 来源
  • 作者

    Yan Mengmeng; Chen Ge; She Yongxin; Ma Jun; Hong Sihui; Shao Yong; Abd El-Aty A. M.; Wang Miao; Wang Shanshan; Wang Jing;

  • 作者单位

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Cairo Univ Fac Vet Med Dept Pharmacol Giza 12211 Egypt;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Inst Qual Stand &

    Testing Technol Agroprod Beijing 100081 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    molecular-imprinted polymer; BNLISA; triazophos; nanozyme; horseradish peroxidase;

    机译：分子印迹聚合物;Bnlisa;三唑酯;纳佐;辣根过氧化物酶;

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