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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Zearalenone Exposure Induces the Apoptosis of Porcine Granulosa Cells and Changes Long Noncoding RNA Expression To Promote Antiapoptosis by Activating the JAK2-STAT3 Pathway
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Zearalenone Exposure Induces the Apoptosis of Porcine Granulosa Cells and Changes Long Noncoding RNA Expression To Promote Antiapoptosis by Activating the JAK2-STAT3 Pathway

机译：Zearalenone暴露诱导猪颗粒细胞的凋亡，并通过激活JAK2-STAT3途径来改变长度非编码RNA表达以促进抗痘病

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摘要

Zearalenone (ZEA), a pathogenic toxin produced by Fusarium, is widely detected in moldy feed materials. Previous studies have reported that ZEA exerts a harmful influence on animal reproductive systems; however, its effects on the changes of long noncoding RNAs (lncRNAs) remain unclear. Here, tackling this question, we performed RNA sequencing on porcine granulosa cells (GCs) after being exposed to 10 and 30 mu M ZEA in vitro. The results showed that ZEA exposure observably changed the expression of lncRNAs in porcine GCs and increased the rate of apoptosis. Furthermore, Gene Ontology analysis showed that ZEA exposure induced variation of the Janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) signaling pathway in porcine GCs. To verify our bioinformatics analysis, western blotting and immunofluorescence analysis were performed and the results demonstrated that porcine GCs after ZEA exposure increased the expression of key proteins in the JAK2-STAT3 signaling pathway. Further bioinformatics analysis found that MSTRG.22680 and MSTRG.23882 played a pivotal role in activating the JAK2-STAT3 signaling pathway. To summarize, our results throw light on the fact that ZEA exposure dramatically increases the apoptosis of porcine GCs and alters the expression of lncRNAs that play an antiapoptotic role in porcine GCs via activating the JAK2-STAT3 signaling pathway.
机译：Zearalenone（ZEA）是由镰刀菌产生的致病性毒素，广泛检测到发霉的饲料材料中。以前的研究报告说，ZEA对动物生殖系统产生有害影响;然而，它对长度非编码RNA（LNCRNA）变化的影响仍不清楚。在这里，在体外暴露于10和30μmZea后，我们在猪颗粒细胞（GCS）上进行RNA测序。结果表明，ZEA暴露可观察地改变了猪GCS中LNCRNA的表达，增加了凋亡率。此外，基因本体论分析表明，ZEA暴露诱导猪GCS中Janus激酶2（JAK2） - 次静电传感器和转录3（STAT3）信号通路的激活剂的变化。为了验证我们的生物信息学分析，进行蛋白质印迹和免疫荧光分析，结果表明ZEA暴露后的猪GCS增加了JAK2-STAT3信号通路中的关键蛋白的表达。进一步的生物信息学分析发现，MSTRG.22680和MSTRG.23882在激活JAK2-STAT3信号通路方面发挥了关键作用。总而言之，我们的结果揭示了Zea暴露显着增加了猪GCS的凋亡，并改变了通过激活JAK2-STAT3信号传导途径在猪GCS中发挥抗曝光作用的LNCRNA的表达。

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