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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis
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Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

机译：由核磁共振代谢组合方法耦合到多变量数据分析的核磁共振amphidinium carterae的腋生和其他感兴趣的生物产物的生产

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摘要

This study assessed the feasibility of an NMR metabolomics approach coupled to multivariate data analysis to monitor the naturally present or stresses-elicited metabolites from a long-term (>170 days) culture of the dinoflagellate marine microalgae Amphidinium carterae grown in a fiberglass paddlewheel-driven raceway photobioreactor. Metabolic contents, in particular, in two members of the amphidinol family, amphidinol A and its 7-sulfate derivative amphidinol B (referred as APDs), and other compounds of interest (fatty acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration levels of the f/2 medium nutrients and daily mean irradiance. Operating with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD production, which was also detected by an increase in hemolytic activity of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral activity measured in the methanolic extracts of the biomass. Increased daily irradiance was accompanied by a general decrease in pigments and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated fatty acids), and DHA (docosahexaenoic acid), while increased nutrient availability lead to an increase in sugar, amino acid, and PUFA omega-3 contents and pigments and a decrease in SFAs and MUFAs. NMR-based metabolomics is shown to be a fast and suitable method to accompany the production of APD and bioactive compounds without the need of tedious isolation methods and bioassays. The two APD compounds were chemically identified by spectroscopic NMR and spectrometric ESI-IT MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF MS (ESI time-of-flight mass spectrometry) methods.
机译：该研究评估了NMR代谢组合方法的可行性，其偶联于多变量数据分析，从长期（> 170天）培养到纤维碎轮驱动的甲霉素碎片的长期（> 170天）培养物中的自然存在或应激引发的代谢物。滚道光生物反应器。通过改变来评估代谢含量在amphidinol家族，amphidinolA及其7-硫酸盐衍生物Amphidinolb（脂肪酸，类胡萝卜素，奥氧化物等）的两个成员中进行评估浓度水平的F / 2中营养物和每日平均辐照度。使用24小时正弦光循环操作，允许APD生产增加3倍，这也通过增加A.Carterae生物量的甲醇提取物的溶血活性的增加来检测。 APDS的存在与在生物质的甲醇溶液中测量的抗肿瘤活性一致。每日辐照度增加伴有颜料的一般性降低和SFAs（饱和脂肪酸），mufas（Monounsposated脂肪酸）和DHA（十二磺酰己酸）的增加，同时增加营养可用性导致糖，氨基酸增加，和Pufa Omega-3含量和颜料和SFA和MUFA的减少。基于NMR的代谢组科是一种快速且合适的方法，其伴随APD和生物活性化合物的产生而不需要繁琐的分离方法和生物测定。通过光谱NMR和光谱ESI-IT MS（电喷雾电离离子捕集物质谱法）和ESI-TOF MS（ESI飞行时间质谱）方法化学鉴定了两种APD化合物。

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