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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells
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Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells

机译：juglone诱导的细胞周期停滞和细胞凋亡在Ishikawa人子宫内膜癌细胞中的机制

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摘要

The molecular mechanism of Juglone-induced cell cycle arrest and apoptosis in human endometrial cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 mu M, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein (p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, CHK1, and cyclin A. The expression of Bcl-2 and Bc1-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural anticancer agent.
机译：研究了Juglone诱导的细胞周期停滞和人子宫内膜细胞细胞凋亡的分子机制。 从Carya Cathayensis Sarg的绿色稻壳中纯化Juglone，并通过HPLC，LC-MS / MS和NMR鉴定。 在20.81亩m的IC50，Juglone显着抑制了Ishikawa细胞增殖，如通过对细胞周期蛋白的灭活介导的S期逮捕所示（P <0.05）所示。 暴露于juglone后，ROS水平显着增加，该juglone暴露于p21的mRNA和蛋白表达的并联增加，并降低CDK2，CDC25a，CHK1和细胞周期蛋白A的水平。BCL-2和BC1-XL的表达显着下降 - 再次调节，虽然蟾蜍，坏和细胞c的表达上调，我们以后证实了线粒体途径在Juglone诱导的细胞凋亡中的参与。 我们的体外结果表明，Juglone可以进一步研究一种有效的天然抗癌剂。

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