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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Isolation and Identification of Aryl Hydrocarbon Receptor Modulators in White Button Mushrooms (Agaricus bisporus)
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Isolation and Identification of Aryl Hydrocarbon Receptor Modulators in White Button Mushrooms (Agaricus bisporus)

机译：白色按钮蘑菇中芳基烃受体调节剂的分离与鉴定（姬松茸）

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摘要

Natural aryl hydrocarbon (AHR) ligands have been identified in food and herbal medicines, and they may exhibit beneficial activity in humans. In this study, white button (WB) feeding significantly decreased AHR target gene expression in the small intestine of both conventional and germ-free mice. High-performance liquid chromatography (HPLC) fractionation and ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) combined with an AHR-responsive cell-based luciferase gene reporter assay were used to isolate and characterize benzothiazole (BT) derivatives and 6-methylisoquinoline (6-MIQ) as AHR modulators from WB mushrooms. The study showed dose-dependent changes of AHR transformation determined by the cell-based luciferase gene reporter assay and transcription of CYPIA1 in human Caco-2 cells by BT derivatives and 6-MIQ These findings suggested that WB mushroom contains new classes of natural AHR modulators and demonstrated HPLC fractionation and UHPLC-MS/MS combined with a cell-based luciferase gene reporter assay as a useful approach for isolation and characterization of the previously unidentifed AHR modulators from natural products.
机译：已在食品和草药中鉴定天然芳基烃（AHR）配体，它们可能在人类中表现出有益活性。在本研究中，白色按钮（WB）饲料在常规和无菌小鼠的小肠中显着降低了AHR靶基因表达。高效液相色谱（HPLC）分馏和超高效液相色谱串联质谱（UHPLC-MS / MS）与AHR响应细胞的荧光素酶基因报告结果分离并表征苯并噻唑（BT）衍生物和6-甲基异喹啉（6-MIQ）作为来自WB蘑菇的AHR调节剂。该研究表明，由基于细胞的荧光素酶基因报告计测定测定的AHR转化的剂量依赖性变化，并通过BT衍生物和6-MIQ在人CaCO-2细胞中的Cypia1转录这些研究结果表明WB蘑菇含有新的天然AHR调制器类别并证明了HPLC分级和UHPLC-MS / MS与基于细胞的荧光素酶基因报告结果，作为从天然产物中分离和表征以前未征收的AHR调节剂的有用方法。

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