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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina
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Role of Adenosine Monophosphate Deaminase during Fatty Acid Accumulation in Oleaginous Fungus Mortierella alpina

机译：甲苯胺单磷酸末端酶在酸菌菌脂肪酸积累期间的作用

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摘要

In oleaginous micro-organisms, nitrogen limitation activates adenosine monophosphate deaminase (AMPD) and promotes lipogenesis via the inhibition of isocitrate dehydrogenase. We found that the overexpression of homologous AMPD in Mortierella alpina favored lipid synthesis over cell growth. Total fatty acid content in the recombinant strain was 15.0-34.3% higher than that in the control, even though their biomass was similar. During the early fermentation stage, the intracellular AMP level reduced by 40-60%, together with a 1.9-2.7-fold increase in citrate content compared with the control, therefore provided more precursors for fatty acid synthesis. Moreover, the decreased AMP level resulted in metabolic reprogramming, reflected by the blocked TCA cycle and reduction of amino acids, distributing more carbon to lipid synthesis pathways. By coupling the energy balance with lipogenesis, this study provides new insights into cell metabolism under nitrogen-limited conditions and targets the regulation of fatty acid accumulation in oleaginous micro-organisms.
机译：在含油微生物中，氮限制激活腺苷一磷酸脱氨酶（AMPD）并通过抑制异柠檬酸脱氢酶来促进脂肪生成。我们发现，Mortierella Alpina的同源AMPD的过度表达有利于细胞生长的脂质合成。即使它们的生物质相似，重组菌株中的总脂肪酸含量比对照的总脂肪酸含量高出15.0-34.3％。在早期发酵阶段，细胞内AMP水平降低40-60％，与对照相比，柠檬酸含量的增加1.9-2.7倍，因此提供了更多的前体用于脂肪酸合成。此外，降低的AMP水平导致代谢重编程，由封闭的TCA循环和氨基酸还原反映，将更多碳分配给脂质合成途径。通过将能量平衡与脂肪生成耦合，本研究在氮气限制条件下对细胞代谢的新见解，并针对含油微生物的脂肪酸积累的调节。

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