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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Boiling Licorice Produces Self-Assembled Protein Nanoparticles: A Novel Source of Bioactive Nanomaterials
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Boiling Licorice Produces Self-Assembled Protein Nanoparticles: A Novel Source of Bioactive Nanomaterials

机译：沸腾的甘草生产自组装蛋白纳米粒子：一种新型生物活性纳米材料来源

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摘要

As a popular ingredient for western and traditional Chinese medicine, the root and rhizome of Chinese licorice (Glycyrrhiza uralensis Fisch.) is often administered in the form of a decoction. The protein nanoparticles (NPs) self-assembled during the process of decoction. A major constitutive protein (GLP) was purified and determined to have a molecular weight of 28 kDa with an N-terminal sequence of NPDGL IACYC GQYCW. Over 80% of the purified GLP self-assembled into spherical NPs with diameters of 74.1 +/- 0.7 nm and zeta-potential of -24.3 +/- 1.7 mV when boiled in Tris-HC1 buffer (pH = 7.9, 20 mM) at 100 degrees C for 60 min. Each nanoparticle was estimated by the SEC-MALLS approach to be composed of approximately 23 protein molecules. The NPs and GLP showed low cellular toxicity upon four types of cells including MDCK, L-02, HepG2, and Caco2 cells, while the NPs promoted proliferation of normal hepatocytes by 67%. The NPs solubilized the insoluble astragaloside IV by encapsulation. The results suggest a great potential for GLP-NPs as a promising prototype of a type of drug vehicle, a novel source of bioactive nanomaterials from herbal proteins, as well as a new mode of function with herbal components.
机译：作为西方和中医的普遍成分，中国甘草的根和根茎（Glycyrrhiza Uralensis Fisch。）通常以汤剂的形式给药。在煎汤过程中自组装的蛋白质纳米颗粒（NPS）。纯化主要组成蛋白（GLP）并测定分子量为28kDa，N-末端序列的NPDGL IACYC GQYCW。超过80％的纯化GLP自组装成球形NPS，直径为74.1 +/- 0.7nm和Zeta-poly，当在Tris-HCl缓冲液中煮沸时 - 24.3 +/- 1.7 mV（pH = 7.9,20 mm） 100摄氏度60分钟。通过Sec-Malls方法估计每个纳米粒子，其由大约23个蛋白质分子组成。 NPS和GLP在包括MDCK，L-02，HepG2和CaCO 2细胞的四种细胞上显示出低细胞毒性，而NPS将正常肝细胞的增殖促进67％。 NPS通过包封溶解不溶性黄芪IV。结果表明GLP-NPS的巨大潜力作为一种药物载体的有希望的原型，一种来自草药蛋白的新型生物活性纳米材料来源，以及具有草药成分的新功能模式。

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