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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Biofilm Polysaccharide Display Platform: A Natural, Renewable, and Biocompatible Material for Improved Lipase Performance
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Biofilm Polysaccharide Display Platform: A Natural, Renewable, and Biocompatible Material for Improved Lipase Performance

机译：生物膜多糖显示平台：一种用于改善脂肪酶性能的天然，可再生和生物相容性材料

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摘要

Most of the microorganisms can form biofilms, which makes biofilms an abundant bioresource to be exploited. Due to the limitations of the application of current immobilization methods for biofilms, we developed an immobilization method called the biofilm polysaccharide display (BPD) strategy while maintaining the native biofilm structure and catalytic microenvironment of Clostridium acetobutylicum B3. Lipase Lip181 showed significant improvements in stability after chemical immobilization. For example, immobilized Lip181 retained 74.23% of its original activity after incubation for 14 days, while free Lip181 was totally deactivated. In addition, immobilized Lip181 maintained high residual activity (pH 5.0-11.0), which showed improved resistance to pH changes. Notably, this method did not decrease but slightly increased the relative activity of Lip181 from 6.39 to 6.78 U/mg. Immobilized Lip181 was used to prepare cinnamyl acetate, and it showed a maximum yield of 85.09%. Overall, this biofilm immobilization method may promote the development of biocatalytic and biofilm materials.
机译：大多数微生物可以形成生物膜，这使得生物膜成为一个丰富的生物源被利用。由于生物膜的电流固定方法应用的局限性，我们开发了一种称为生物膜多糖显示（BPD）策略的固定方法，同时保持天然生物膜结构和乙酰苯乙烯丁基B3的催化微环境。脂肪酶LIP181显示出化学固定化后稳定性的显着改善。例如，固定化润滑润滑唇181在孵育后保留了74.23％的其原始活性14天，而自由润唇润唇缘完全停用。此外，固定化的润唇润唇缘181保持高残留活性（pH5.0-11.0），其显示出改善的pH值抗性变化。值得注意的是，这种方法没有减少，但略微增加了唇唇181的相对活性从6.39到6.78 u / mg。固定化的润唇脂181用于制备糖醋酸糖，其最大收率为85.09％。总体而言，这种生物膜固定化方法可以促进生物催化和生物膜材料的发育。

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  • 作者

    Dong Hao; Zhang Wenxue; Wang Yibing; Liu Dong; Wang Ping;

  • 作者单位

    East China Univ Sci &

    Technol Sch Biotechnol Shanghai Collaborat Innovat Ctr Biomfg Technol State Key Lab Bioreactor Engn Biomed Nanotechnol Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol Sch Biotechnol Shanghai Collaborat Innovat Ctr Biomfg Technol State Key Lab Bioreactor Engn Biomed Nanotechnol Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol Sch Biotechnol Shanghai Collaborat Innovat Ctr Biomfg Technol State Key Lab Bioreactor Engn Biomed Nanotechnol Shanghai 200237 Peoples R China;

    Nanjing Tech Univ Natl Engn Res Ctr Biotechnol Coll Biotechnol &

    Pharmaceut Engn Nanjing 211816 Peoples R China;

    Univ Minnesota Dept Bioprod &

    Biosyst Engn St Paul MN 55108 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 营养卫生、食品卫生;农业科学;
  • 关键词

    immobilization; biofilm; Clostridium acetobutylicum; lipase; cinnamyl acetate;

    机译：固定化;生物膜;乙酸梭菌;脂肪酶;醋酸糖;

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